3CAE | pdb_00003cae

Structure of NNQQNY as an insert in T7 endonuclease I

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d3caea_	Alpha and beta proteins (a/b)	Restriction endonuclease-like	Restriction endonuclease-like	Endonuclease I (Holliday junction resolvase)	automated matches	(Escherichia phage T7 ) [TaxId: 10760 ], (Escherichia phage T7 ) [TaxId: 10760 ],	SCOPe (2.08)
B	d3caeb_	Alpha and beta proteins (a/b)	Restriction endonuclease-like	Restriction endonuclease-like	Endonuclease I (Holliday junction resolvase)	automated matches	(Escherichia phage T7 ) [TaxId: 10760 ], (Escherichia phage T7 ) [TaxId: 10760 ],	SCOPe (2.08)
C	d3caec_	Alpha and beta proteins (a/b)	Restriction endonuclease-like	Restriction endonuclease-like	Endonuclease I (Holliday junction resolvase)	automated matches	(Escherichia phage T7 ) [TaxId: 10760 ], (Escherichia phage T7 ) [TaxId: 10760 ],	SCOPe (2.08)
D	d3caed_	Alpha and beta proteins (a/b)	Restriction endonuclease-like	Restriction endonuclease-like	Endonuclease I (Holliday junction resolvase)	automated matches	(Escherichia phage T7 ) [TaxId: 10760 ], (Escherichia phage T7 ) [TaxId: 10760 ],	SCOPe (2.08)
E	d3caee_	Alpha and beta proteins (a/b)	Restriction endonuclease-like	Restriction endonuclease-like	Endonuclease I (Holliday junction resolvase)	automated matches	(Escherichia phage T7 ) [TaxId: 10760 ], (Escherichia phage T7 ) [TaxId: 10760 ],	SCOPe (2.08)
F	d3caef_	Alpha and beta proteins (a/b)	Restriction endonuclease-like	Restriction endonuclease-like	Endonuclease I (Holliday junction resolvase)	automated matches	(Escherichia phage T7 ) [TaxId: 10760 ], (Escherichia phage T7 ) [TaxId: 10760 ],	SCOPe (2.08)
H	d3caeh_	Alpha and beta proteins (a/b)	Restriction endonuclease-like	Restriction endonuclease-like	Endonuclease I (Holliday junction resolvase)	automated matches	(Escherichia phage T7 ) [TaxId: 10760 ], (Escherichia phage T7 ) [TaxId: 10760 ],	SCOPe (2.08)
I	d3caei_	Alpha and beta proteins (a/b)	Restriction endonuclease-like	Restriction endonuclease-like	Endonuclease I (Holliday junction resolvase)	automated matches	(Escherichia phage T7 ) [TaxId: 10760 ], (Escherichia phage T7 ) [TaxId: 10760 ],	SCOPe (2.08)
J	d3caej_	Alpha and beta proteins (a/b)	Restriction endonuclease-like	Restriction endonuclease-like	Endonuclease I (Holliday junction resolvase)	automated matches	(Escherichia phage T7 ) [TaxId: 10760 ], (Escherichia phage T7 ) [TaxId: 10760 ],	SCOPe (2.08)
G	d3caeg_	Alpha and beta proteins (a/b)	Restriction endonuclease-like	Restriction endonuclease-like	Endonuclease I (Holliday junction resolvase)	automated matches	(Escherichia phage T7 ) [TaxId: 10760 ], (Escherichia phage T7 ) [TaxId: 10760 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Restriction endonuclease-like	8041518	3000104	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Restriction endonuclease-like	8041518	3000104	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	Restriction endonuclease-like	8041518	3000104	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	Restriction endonuclease-like	8041518	3000104	SCOP2B (2022-06-29)
E	SCOP2B Superfamily	Restriction endonuclease-like	8041518	3000104	SCOP2B (2022-06-29)
F	SCOP2B Superfamily	Restriction endonuclease-like	8041518	3000104	SCOP2B (2022-06-29)
H	SCOP2B Superfamily	Restriction endonuclease-like	8041518	3000104	SCOP2B (2022-06-29)
I	SCOP2B Superfamily	Restriction endonuclease-like	8041518	3000104	SCOP2B (2022-06-29)
J	SCOP2B Superfamily	Restriction endonuclease-like	8041518	3000104	SCOP2B (2022-06-29)
G	SCOP2B Superfamily	Restriction endonuclease-like	8041518	3000104	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Phage_endo_I	e3caeA1	A: a/b three-layered sandwiches	X: Restriction endonuclease-like	H: Restriction endonuclease-like (From Topology)	T: Restriction endonuclease-like	F: Phage_endo_I	ECOD (1.6)
B	Phage_endo_I	e3caeB1	A: a/b three-layered sandwiches	X: Restriction endonuclease-like	H: Restriction endonuclease-like (From Topology)	T: Restriction endonuclease-like	F: Phage_endo_I	ECOD (1.6)
C	Phage_endo_I	e3caeC1	A: a/b three-layered sandwiches	X: Restriction endonuclease-like	H: Restriction endonuclease-like (From Topology)	T: Restriction endonuclease-like	F: Phage_endo_I	ECOD (1.6)
D	Phage_endo_I	e3caeD1	A: a/b three-layered sandwiches	X: Restriction endonuclease-like	H: Restriction endonuclease-like (From Topology)	T: Restriction endonuclease-like	F: Phage_endo_I	ECOD (1.6)
E	Phage_endo_I	e3caeE1	A: a/b three-layered sandwiches	X: Restriction endonuclease-like	H: Restriction endonuclease-like (From Topology)	T: Restriction endonuclease-like	F: Phage_endo_I	ECOD (1.6)
F	Phage_endo_I	e3caeF1	A: a/b three-layered sandwiches	X: Restriction endonuclease-like	H: Restriction endonuclease-like (From Topology)	T: Restriction endonuclease-like	F: Phage_endo_I	ECOD (1.6)
H	Phage_endo_I	e3caeH1	A: a/b three-layered sandwiches	X: Restriction endonuclease-like	H: Restriction endonuclease-like (From Topology)	T: Restriction endonuclease-like	F: Phage_endo_I	ECOD (1.6)
I	Phage_endo_I	e3caeI1	A: a/b three-layered sandwiches	X: Restriction endonuclease-like	H: Restriction endonuclease-like (From Topology)	T: Restriction endonuclease-like	F: Phage_endo_I	ECOD (1.6)
J	Phage_endo_I	e3caeJ1	A: a/b three-layered sandwiches	X: Restriction endonuclease-like	H: Restriction endonuclease-like (From Topology)	T: Restriction endonuclease-like	F: Phage_endo_I	ECOD (1.6)
G	Phage_endo_I	e3caeG1	A: a/b three-layered sandwiches	X: Restriction endonuclease-like	H: Restriction endonuclease-like (From Topology)	T: Restriction endonuclease-like	F: Phage_endo_I	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.40.91.30	Alpha Beta	3-Layer(aba) Sandwich	Restriction Endonuclease		CATH (4.3.0)
B	3.40.91.30	Alpha Beta	3-Layer(aba) Sandwich	Restriction Endonuclease		CATH (4.3.0)
C	3.40.91.30	Alpha Beta	3-Layer(aba) Sandwich	Restriction Endonuclease		CATH (4.3.0)
D	3.40.91.30	Alpha Beta	3-Layer(aba) Sandwich	Restriction Endonuclease		CATH (4.3.0)
E	3.40.91.30	Alpha Beta	3-Layer(aba) Sandwich	Restriction Endonuclease		CATH (4.3.0)
F	3.40.91.30	Alpha Beta	3-Layer(aba) Sandwich	Restriction Endonuclease		CATH (4.3.0)
H	3.40.91.30	Alpha Beta	3-Layer(aba) Sandwich	Restriction Endonuclease		CATH (4.3.0)
I	3.40.91.30	Alpha Beta	3-Layer(aba) Sandwich	Restriction Endonuclease		CATH (4.3.0)
J	3.40.91.30	Alpha Beta	3-Layer(aba) Sandwich	Restriction Endonuclease		CATH (4.3.0)
G	3.40.91.30	Alpha Beta	3-Layer(aba) Sandwich	Restriction Endonuclease		CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D, E A, B, C, D, E, F, G, H, I, J	PF05367	Phage endonuclease I (Phage_endo_I)	Phage endonuclease I	The bacteriophage endonuclease I is a nuclease that is selective for the structure of the four-way Holliday DNA junction [1].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D, E A, B, C, D, E, F, G, H, I, J	Endonuclease I	DNA endonuclease activity endonuclease activity hydrolase activity, acting on ester bonds catalytic activity, acting on DNA nuclease activity catalytic activity, acting on a nucleic acid hydrolase activity double-stranded DNA endonuclease activity catalytic activity DNA nuclease activity DNA binding binding nucleic acid binding crossover junction DNA endonuclease activity DNA endonuclease activity endonuclease activity hydrolase activity, acting on ester bonds catalytic activity, acting on DNA nuclease activity catalytic activity, acting on a nucleic acid hydrolase activity double-stranded DNA endonuclease activity catalytic activity DNA nuclease activity DNA binding binding nucleic acid binding crossover junction DNA endonuclease activity DNA endonuclease activity, producing 3'-phosphomonoesters DNA endonuclease activity, producing 5'-phosphomonoesters deoxyribonuclease IV (phage-T4-induced) activity	symbiont-mediated perturbation of host cellular process symbiont-mediated suppression of host gene expression biological process involved in interspecies interaction between organisms symbiont-mediated perturbation of host process symbiont-mediated perturbation of host gene expression modulation of process of another organism biological process involved in interaction with host biological process involved in symbiotic interaction nucleobase-containing compound metabolic process metabolic process cellular process primary metabolic process DNA metabolic process macromolecule metabolic process symbiont-mediated perturbation of host cellular process symbiont-mediated suppression of host gene expression biological process involved in interspecies interaction between organisms symbiont-mediated perturbation of host process symbiont-mediated perturbation of host gene expression modulation of process of another organism biological process involved in interaction with host biological process involved in symbiotic interaction nucleobase-containing compound metabolic process metabolic process cellular process primary metabolic process DNA metabolic process macromolecule metabolic process DNA integration nucleic acid metabolic process viral DNA genome replication viral life cycle nucleobase-containing compound biosynthetic process macromolecule biosynthetic process degradation of host chromosome by virus nucleic acid biosynthetic process biosynthetic process viral genome replication viral process DNA biosynthetic process	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D, E A, B, C, D, E, F, G, H, I, J	IPR008029	Bacteriophage T7, Gp3, endodeoxynuclease I	Family
A, B, C, D, E A, B, C, D, E, F, G, H, I, J	IPR011335	Restriction endonuclease type II-like	Homologous Superfamily

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.