5DSG

Structure of the M4 muscarinic acetylcholine receptor (M4-mT4L) bound to tiotropium

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations
Membrane Protein Annotation: OPM
Membrane Protein Annotation: PDBTM
Membrane Protein Annotation: mpstruc

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
B	SCOP2B Superfamily	G protein-coupled receptor-like	8091156	3000473	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	G protein-coupled receptor-like	8091156	3000473	SCOP2B (2022-06-29)
A	SCOP2 Family	Class A (rhodopsin) G protein-coupled receptor-like	8091155	4001858	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	G protein-coupled receptor-like	8091156	3000473	SCOP2 (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
B	7tm_1_2	e5dsgB1	A: alpha bundles	X: Family A G protein-coupled receptor-like (From Topology)	H: Family A G protein-coupled receptor-like (From Topology)	T: Family A G protein-coupled receptor-like	F: 7tm_1_2	ECOD (1.6)
B	Phage_lysozyme_2	e5dsgB2	A: a+b complex topology	X: Lysozyme-like (From Homology)	H: Lysozyme-like	T: Lysozyme-like	F: Phage_lysozyme_2	ECOD (1.6)
A	7tm_1_2	e5dsgA1	A: alpha bundles	X: Family A G protein-coupled receptor-like (From Topology)	H: Family A G protein-coupled receptor-like (From Topology)	T: Family A G protein-coupled receptor-like	F: 7tm_1_2	ECOD (1.6)
A	Phage_lysozyme_2	e5dsgA2	A: a+b complex topology	X: Lysozyme-like (From Homology)	H: Lysozyme-like	T: Lysozyme-like	F: Phage_lysozyme_2	ECOD (1.6)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF00959	Phage lysozyme (Phage_lysozyme)	Phage lysozyme	This family includes lambda phage lysozyme and E. coli endolysin.	Domain
A, B	PF00001	7 transmembrane receptor (rhodopsin family) (7tm_1)	7 transmembrane receptor (rhodopsin family)	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	Muscarinic acetylcholine receptor M4,Endolysin,Endolysin,Muscarinic acetylcholine receptor M4	neurotransmitter receptor activity G protein-coupled amine receptor activity G protein-coupled acetylcholine receptor activity postsynaptic neurotransmitter receptor activity molecular transducer activity G protein-coupled receptor activity signaling receptor activity transmembrane signaling receptor activity G protein-coupled neurotransmitter receptor activity acetylcholine receptor activity G protein-coupled serotonin receptor activity hydrolase activity, acting on glycosyl bonds peptidoglycan muralytic activity hydrolase activity, hydrolyzing O-glycosyl compounds neurotransmitter receptor activity G protein-coupled amine receptor activity G protein-coupled acetylcholine receptor activity postsynaptic neurotransmitter receptor activity molecular transducer activity G protein-coupled receptor activity signaling receptor activity transmembrane signaling receptor activity G protein-coupled neurotransmitter receptor activity acetylcholine receptor activity G protein-coupled serotonin receptor activity hydrolase activity, acting on glycosyl bonds peptidoglycan muralytic activity hydrolase activity, hydrolyzing O-glycosyl compounds lysozyme activity hydrolase activity catalytic activity	cellular response to stimulus cellular response to nitrogen compound regulation of cellular process response to stimulus signaling cell communication synaptic signaling signal transduction cellular response to acetylcholine regulation of biological process biological regulation response to oxygen-containing compound cell-cell signaling response to nitrogen compound cellular response to stimulus cellular response to nitrogen compound regulation of cellular process response to stimulus signaling cell communication synaptic signaling signal transduction cellular response to acetylcholine regulation of biological process biological regulation response to oxygen-containing compound cell-cell signaling response to nitrogen compound acetylcholine receptor signaling pathway postsynaptic signal transduction cellular response to oxygen-containing compound adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway cellular response to chemical stimulus G protein-coupled acetylcholine receptor signaling pathway response to chemical adenylate cyclase-modulating G protein-coupled receptor signaling pathway response to acetylcholine G protein-coupled receptor signaling pathway cellular process adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway cell surface receptor signaling pathway trans-synaptic signaling anterograde trans-synaptic signaling chemical synaptic transmission regulation of locomotion locomotion G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger biological process involved in interspecies interaction between organisms response to other organism defense response response to external stimulus response to biotic stimulus defense response to bacterium defense response to other organism response to stress response to bacterium response to external biotic stimulus carbohydrate derivative catabolic process catabolic process peptidoglycan catabolic process glycosaminoglycan metabolic process carbohydrate derivative metabolic process aminoglycan catabolic process macromolecule catabolic process glycosaminoglycan catabolic process aminoglycan metabolic process peptidoglycan metabolic process metabolic process macromolecule metabolic process symbiont-mediated cytolysis of host cell viral life cycle disruption of cell in another organism cytolysis biological process involved in interaction with host biological process involved in symbiotic interaction viral release from host cell by cytolysis disruption of cellular anatomical structure in another organism exit from host cell viral process cytolysis in another organism viral release from host cell disruption of anatomical structure in another organism symbiont-mediated killing of host cell killing of cells of another organism cell killing disruption of plasma membrane integrity in another organism cell wall organization or biogenesis cell wall macromolecule catabolic process cell wall macromolecule metabolic process	cell periphery postsynapse cellular anatomical structure postsynaptic membrane synapse cell junction membrane plasma membrane plasma membrane region synaptic membrane somatodendritic compartment cell projection dendrite dendritic tree cell periphery postsynapse cellular anatomical structure postsynaptic membrane synapse cell junction membrane plasma membrane plasma membrane region synaptic membrane somatodendritic compartment cell projection dendrite dendritic tree neuron projection plasma membrane bounded cell projection host cellular component host intracellular part host cell cytoplasm host cell part host intracellular region

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR001432	Muscarinic acetylcholine receptor M4	Family
A, B	IPR000995	Muscarinic acetylcholine receptor family	Family
A, B	IPR000276	G protein-coupled receptor, rhodopsin-like	Family
A, B	IPR017452	GPCR, rhodopsin-like, 7TM	Domain
A, B	IPR052619	Bacteriophage lysozyme-like	Family
A, B	IPR001165	T4-type lysozyme	Family
A, B	IPR034690	Endolysin T4 type	Family
A, B	IPR002196	Glycoside hydrolase, family 24	Family
A, B	IPR023347	Lysozyme domain superfamily	Homologous Superfamily
A, B	IPR023346	Lysozyme-like domain superfamily	Homologous Superfamily

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A, B	Pharos: P08173	Catatonia osteosarcoma peripheral arterial disease psoriasis schizophrenia biliary dyskinesia diabetes mellitus anorexia nervosa endogenous depression gastric ulcer peptic ulcer disease autism spectrum disorder chronic obstructive pulmonary disease

Membrane Protein Annotation: OPM OPM Database Homepage

Chains	External Link	Type	Class	Superfamily	Family
A, B	OPM	Transmembrane	Alpha-helical polytopic	Rhodopsin-like receptors and pumps	G-protein coupled receptors, family A

Membrane Protein Annotation: PDBTM PDBTM Database Homepage

Chains	External Link	Comment
A, B	PDBTM	Annotated as membrane protein by PDBTM

Membrane Protein Annotation: mpstruc mpstruc Database Homepage

Chains	Resource Link	Group	Subgroup	Protein
A, B	mpstruc	TRANSMEMBRANE PROTEINS: ALPHA-HELICAL	G Protein-Coupled Receptors: Class A	M4 muscarinic acetylcholine receptor with bound tiotropium

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.