5ZKC

Crystal structure of rationally thermostabilized M2 muscarinic acetylcholine receptor bound with NMS

External Resource: Annotation

Domain Annotation: ECOD Classification
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations
Membrane Protein Annotation: OPM
Membrane Protein Annotation: PDBTM
Membrane Protein Annotation: MemProtMD

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	7tm_1_2	e5zkcA2	A: alpha bundles	X: Family A G protein-coupled receptor-like (From Topology)	H: Family A G protein-coupled receptor-like (From Topology)	T: Family A G protein-coupled receptor-like	F: 7tm_1_2	ECOD (1.6)
A	Cytochrom_B562_1	e5zkcA1	A: a+b complex topology	X: Lysozyme-like (From Homology)	H: Lysozyme-like	T: Lysozyme-like	F: Cytochrom_B562_1	ECOD (1.6)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF07361	Cytochrome b562 (Cytochrom_B562)	Cytochrome b562	-	Family
A	PF00001	7 transmembrane receptor (rhodopsin family) (7tm_1)	7 transmembrane receptor (rhodopsin family)	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	Muscarinic acetylcholine receptor M2,Apo-cytochrome b562,Muscarinic acetylcholine receptor M2	binding organic cyclic compound binding tetrapyrrole binding heme binding electron transfer activity cation binding transition metal ion binding iron ion binding ion binding metal ion binding small molecule binding protein binding arrestin family protein binding neurotransmitter receptor activity binding organic cyclic compound binding tetrapyrrole binding heme binding electron transfer activity cation binding transition metal ion binding iron ion binding ion binding metal ion binding small molecule binding protein binding arrestin family protein binding neurotransmitter receptor activity G protein-coupled amine receptor activity G protein-coupled acetylcholine receptor activity postsynaptic neurotransmitter receptor activity molecular transducer activity G protein-coupled receptor activity signaling receptor activity transmembrane signaling receptor activity G protein-coupled neurotransmitter receptor activity acetylcholine receptor activity G protein-coupled serotonin receptor activity	electron transport chain metabolic process cellular process generation of precursor metabolites and energy heart process multicellular organismal process system process regulation of biological process regulation of heart contraction regulation of system process biological regulation circulatory system process regulation of blood circulation regulation of multicellular organismal process electron transport chain metabolic process cellular process generation of precursor metabolites and energy heart process multicellular organismal process system process regulation of biological process regulation of heart contraction regulation of system process biological regulation circulatory system process regulation of blood circulation regulation of multicellular organismal process heart contraction blood circulation cellular response to stimulus cellular response to nitrogen compound regulation of cellular process response to stimulus signaling cell communication synaptic signaling signal transduction cellular response to acetylcholine response to oxygen-containing compound cell-cell signaling response to nitrogen compound acetylcholine receptor signaling pathway postsynaptic signal transduction cellular response to oxygen-containing compound adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway cellular response to chemical stimulus G protein-coupled acetylcholine receptor signaling pathway response to chemical adenylate cyclase-modulating G protein-coupled receptor signaling pathway response to acetylcholine G protein-coupled receptor signaling pathway adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway trans-synaptic signaling regulation of signaling modulation of chemical synaptic transmission presynaptic modulation of chemical synaptic transmission chemical synaptic transmission regulation of trans-synaptic signaling anterograde trans-synaptic signaling regulation of cell communication phospholipase C-activating G protein-coupled receptor signaling pathway phospholipase C-activating G protein-coupled acetylcholine receptor signaling pathway muscle contraction muscle system process regulation of muscle system process smooth muscle contraction regulation of smooth muscle contraction regulation of muscle contraction developmental process multicellular organism development anatomical structure development nervous system development system development biological process involved in interspecies interaction between organisms response to other organism response to external stimulus response to external biotic stimulus response to virus response to biotic stimulus G protein-coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger	cellular anatomical structure periplasmic space symmetric synapse neuron to neuron synapse synapse cell junction cell periphery postsynapse postsynaptic membrane membrane plasma membrane plasma membrane region synaptic membrane membrane-bounded organelle cellular anatomical structure periplasmic space symmetric synapse neuron to neuron synapse synapse cell junction cell periphery postsynapse postsynaptic membrane membrane plasma membrane plasma membrane region synaptic membrane membrane-bounded organelle intracellular anatomical structure intracellular vesicle intracellular organelle bounding membrane of organelle cytoplasmic vesicle vesicle membrane clathrin-coated vesicle endocytic vesicle membrane coated vesicle vesicle endocytic vesicle clathrin-coated vesicle membrane clathrin-coated endocytic vesicle membrane intracellular membrane-bounded organelle clathrin-coated endocytic vesicle cytoplasmic vesicle membrane coated vesicle membrane organelle cytoplasm organelle membrane presynapse presynaptic membrane somatodendritic compartment cell projection dendrite dendritic tree neuron projection plasma membrane bounded cell projection cholinergic synapse glutamatergic synapse cell body neuronal cell body asymmetric synapse axon axon terminus distal axon neuron projection terminus

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR010980	Cytochrome c/b562	Homologous Superfamily
A	IPR009155	Cytochrome b562	Family
A	IPR000995	Muscarinic acetylcholine receptor family	Family
A	IPR000276	G protein-coupled receptor, rhodopsin-like	Family
A	IPR017452	GPCR, rhodopsin-like, 7TM	Domain
A	IPR001065	Muscarinic acetylcholine receptor M2	Family

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A	Pharos: P08172	diabetic polyneuropathy epilepsy Bradycardia diabetic autonomic neuropathy diabetic neuropathy alcohol abuse substance dependence biliary dyskinesia gastric ulcer large cell medulloblastoma brain injury depressive disorder schizophrenia substance abuse bipolar disorder diabetic polyneuropathy epilepsy Bradycardia diabetic autonomic neuropathy diabetic neuropathy alcohol abuse substance dependence biliary dyskinesia gastric ulcer large cell medulloblastoma brain injury depressive disorder schizophrenia substance abuse bipolar disorder major depressive disorder peptic ulcer disease psoriasis drug dependence substance-related disorder type 1 diabetes mellitus malignant colon neoplasm chronic obstructive pulmonary disease

Membrane Protein Annotation: OPM OPM Database Homepage

Chains	External Link	Type	Class	Superfamily	Family
A	OPM	Transmembrane	Alpha-helical polytopic	Rhodopsin-like receptors and pumps	G-protein coupled receptors, family A

Membrane Protein Annotation: PDBTM PDBTM Database Homepage

Chains	External Link	Comment
A	PDBTM	Annotated as membrane protein by PDBTM

Membrane Protein Annotation: MemProtMD MemProtMD Database Homepage

Chains	External Link	Comment
A	MemProtMD	Annotated as membrane protein by MemProtMD

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.