7E5E

Crystal structure of GDP-bound GNAS in complex with the cyclic peptide inhibitor GD20

External Resource: Annotation

Domain Annotation: ECOD Classification
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations
Protein Modification Annotation

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	G-alpha	e7e5eA1	A: alpha arrays	X: Transducin (alpha subunit), insertion domain (From Topology)	H: Transducin (alpha subunit), insertion domain (From Topology)	T: Transducin (alpha subunit), insertion domain	F: G-alpha	ECOD (1.6)
A	Ras	e7e5eA2	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: Ras	ECOD (1.6)
D	G-alpha	e7e5eD2	A: alpha arrays	X: Transducin (alpha subunit), insertion domain (From Topology)	H: Transducin (alpha subunit), insertion domain (From Topology)	T: Transducin (alpha subunit), insertion domain	F: G-alpha	ECOD (1.6)
D	Ras	e7e5eD1	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: Ras	ECOD (1.6)
B	G-alpha	e7e5eB2	A: alpha arrays	X: Transducin (alpha subunit), insertion domain (From Topology)	H: Transducin (alpha subunit), insertion domain (From Topology)	T: Transducin (alpha subunit), insertion domain	F: G-alpha	ECOD (1.6)
B	Ras	e7e5eB1	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: Ras	ECOD (1.6)
C	G-alpha	e7e5eC1	A: alpha arrays	X: Transducin (alpha subunit), insertion domain (From Topology)	H: Transducin (alpha subunit), insertion domain (From Topology)	T: Transducin (alpha subunit), insertion domain	F: G-alpha	ECOD (1.6)
C	Ras	e7e5eC2	A: a/b three-layered sandwiches	X: P-loop domains-like	H: P-loop domains-related	T: P-loop containing nucleoside triphosphate hydrolases	F: Ras	ECOD (1.6)

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D	Isoform Gnas-2 of Guanine nucleotide-binding protein G(s) subunit alpha isoforms short	binding guanyl nucleotide binding GTP binding nucleotide binding guanyl ribonucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding organic cyclic compound binding purine nucleotide binding anion binding heterocyclic compound binding carbohydrate derivative binding binding guanyl nucleotide binding GTP binding nucleotide binding guanyl ribonucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding ion binding organic cyclic compound binding purine nucleotide binding anion binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding small molecule binding D1 dopamine receptor binding protein binding signaling receptor binding G protein-coupled receptor binding dopamine receptor binding protein-containing complex binding G-protein beta/gamma-subunit complex binding pyrophosphatase activity GTPase activity G protein activity ribonucleoside triphosphate phosphatase activity molecular function regulator activity hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides hydrolase activity hydrolase activity, acting on acid anhydrides catalytic activity enzyme regulator activity cyclase regulator activity enzyme activator activity cyclase activator activity molecular function activator activity adenylate cyclase regulator activity adenylate cyclase activator activity cation binding metal ion binding	developmental process multicellular organismal process epithelium development ectodermal placode morphogenesis epidermis development molting cycle process hair cycle animal organ development hair follicle placode formation tissue development ectodermal placode formation anatomical structure morphogenesis skin development skin epidermis development hair follicle development developmental process multicellular organismal process epithelium development ectodermal placode morphogenesis epidermis development molting cycle process hair cycle animal organ development hair follicle placode formation tissue development ectodermal placode formation anatomical structure morphogenesis skin development skin epidermis development hair follicle development molting cycle anatomical structure development anatomical structure formation involved in morphogenesis ectodermal placode development hair cycle process system process sensory perception of chemical stimulus sensory perception nervous system process sensory perception of smell cellular response to nitrogen compound cellular response to stimulus response to stimulus response to endogenous stimulus response to peptide hormone cellular response to peptide hormone stimulus response to oxygen-containing compound response to nitrogen compound response to glucagon cellular response to oxygen-containing compound cellular response to glucagon stimulus cellular response to endogenous stimulus cellular response to chemical stimulus response to hormone response to chemical cellular process cellular response to hormone stimulus multicellular organism development skeletal system development bone development system development regulation of body fluid levels wound healing homotypic cell-cell adhesion cell activation hemostasis biological regulation platelet aggregation blood coagulation cell adhesion cell-cell adhesion response to stress coagulation regulation of biological quality response to wounding platelet activation cognition developmental growth growth response to ketone response to prostaglandin response to lipid response to alcohol cellular response to prostaglandin stimulus cellular response to prostaglandin E stimulus cellular response to alcohol cellular response to lipid response to prostaglandin E cellular response to ketone regulation of biological process regulation of cellular process signaling adenylate cyclase-activating G protein-coupled receptor signaling pathway cell communication adenylate cyclase-modulating G protein-coupled receptor signaling pathway G protein-coupled receptor signaling pathway signal transduction homeostatic process positive regulation of biological process adaptive thermogenesis positive regulation of metabolic process multicellular organismal-level homeostasis regulation of cold-induced thermogenesis cold-induced thermogenesis temperature homeostasis positive regulation of cold-induced thermogenesis positive regulation of multicellular organismal process metabolic process regulation of multicellular organismal process regulation of metabolic process positive regulation of cellular process protein localization regulation of localization establishment of protein localization nitrogen compound transport localization cellular macromolecule localization regulation of peptide hormone secretion regulation of hormone levels establishment of protein localization to extracellular region protein secretion establishment of localization regulation of protein secretion regulation of secretion cellular localization regulation of hormone secretion regulation of protein localization regulation of peptide secretion transport hormone secretion protein localization to extracellular region peptide secretion secretion by cell regulation of secretion by cell amide transport signal release regulation of signaling secretion regulation of transport regulation of peptide transport regulation of establishment of protein localization cell-cell signaling export from cell regulation of protein transport peptide transport protein transport regulation of cellular localization regulation of insulin secretion peptide hormone secretion macromolecule localization hormone transport regulation of cell communication insulin secretion adenylate cyclase-activating adrenergic receptor signaling pathway adrenergic receptor signaling pathway establishment of localization in cell intracellular transport regulation of defense response regulation of response to external stimulus negative regulation of inflammatory response negative regulation of biological process inflammatory response to antigenic stimulus regulation of response to stress regulation of immune system process negative regulation of inflammatory response to antigenic stimulus defense response immune response negative regulation of response to stimulus negative regulation of immune response regulation of inflammatory response to antigenic stimulus response to external stimulus regulation of inflammatory response regulation of response to stimulus negative regulation of immune system process immune system process negative regulation of response to external stimulus negative regulation of defense response regulation of immune response inflammatory response positive regulation of adenylate cyclase activity positive regulation of cyclase activity regulation of catalytic activity positive regulation of catalytic activity positive regulation of molecular function regulation of molecular function regulation of lyase activity activation of adenylate cyclase activity regulation of adenylate cyclase activity regulation of cyclase activity positive regulation of lyase activity response to dopamine cellular response to dopamine response to monoamine response to catecholamine adenylate cyclase-activating dopamine receptor signaling pathway cellular response to catecholamine stimulus response to organic cyclic compound G protein-coupled dopamine receptor signaling pathway cellular response to organic cyclic compound cellular response to monoamine stimulus renal system process chemical homeostasis multicellular organismal-level water homeostasis multicellular organismal-level chemical homeostasis renal water homeostasis	cellular anatomical structure membrane protein-containing complex extrinsic component of plasma membrane catalytic complex plasma membrane protein complex cytoplasmic side of membrane plasma membrane membrane protein complex heterotrimeric G-protein complex side of membrane cell periphery cytoplasmic side of plasma membrane GTPase complex cellular anatomical structure membrane protein-containing complex extrinsic component of plasma membrane catalytic complex plasma membrane protein complex cytoplasmic side of membrane plasma membrane membrane protein complex heterotrimeric G-protein complex side of membrane cell periphery cytoplasmic side of plasma membrane GTPase complex extrinsic component of membrane extrinsic component of cytoplasmic side of plasma membrane extracellular exosome membrane-bounded organelle extracellular vesicle extracellular organelle extracellular region organelle extracellular space vesicle extracellular membrane-bounded organelle intracellular anatomical structure cytoplasm cytosol Golgi apparatus subcompartment Golgi apparatus organelle subcompartment trans-Golgi network membrane intracellular membrane-bounded organelle endomembrane system intracellular organelle trans-Golgi network organelle membrane
E, F, G, H	GD20	-	-	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D	IPR001019	Guanine nucleotide binding protein (G-protein), alpha subunit	Family
A, B, C, D	IPR011025	G protein alpha subunit, helical insertion	Homologous Superfamily
A, B, C, D	IPR027417	P-loop containing nucleoside triphosphate hydrolase	Homologous Superfamily
A, B, C, D	IPR000367	G-protein alpha subunit, group S	Family

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A, B, C, D	Pharos: P63092	McCune-Albright syndrome progressive osseous heteroplasia pseudohypoparathyroidism type 1A pseudohypoparathyroidism polycystic ovary syndrome acromegaly pseudohypoparathyroidism type 1B cholangiocarcinoma pseudopseudohypoparathyroidism precocious puberty obesity disorder substance withdrawal syndrome pseudohypoaldosteronism ACTH-independent macronodular adrenal hyperplasia 1 neuroblastoma McCune-Albright syndrome progressive osseous heteroplasia pseudohypoparathyroidism type 1A pseudohypoparathyroidism polycystic ovary syndrome acromegaly pseudohypoparathyroidism type 1B cholangiocarcinoma pseudopseudohypoparathyroidism precocious puberty obesity disorder substance withdrawal syndrome pseudohypoaldosteronism ACTH-independent macronodular adrenal hyperplasia 1 neuroblastoma brachydactyly intellectual disability pseudohypoparathyroidism type 1C Cafe-au-Lait Spots hypertensive disorder monostotic fibrous dysplasia cancer colorectal cancer intrahepatic cholangiocarcinoma endocrine system disease autosomal dominant pseudohypoaldosteronism type 1 sex cord-stromal tumor Cushing disease due to pituitary adenoma adrenal gland cancer growth hormone-producing pituitary gland adenoma pilocytic astrocytoma tuberculosis interstitial cystitis congenital hypothyroidism large cell medulloblastoma Cushing syndrome malignant glioma acquired metabolic disease progressive supranuclear palsy medulloblastoma pseudohypoaldosteronism type 2 ductal breast carcinoma in situ adenoma adrenal gland hyperfunction osteitis fibrosa ovarian cancer atypical teratoid rhabdoid tumor pancreatic intraductal papillary-mucinous neoplasm with low grade dysplasia bile duct cancer testicular sex cord-stromal neoplasm pituitary cancer craniosynostosis Cushing syndrome due to macronodular adrenal hyperplasia fibrodysplasia ossificans progressiva oligodendroglioma gallstones osteogenesis imperfecta schizophrenia growth hormone secreting pituitary adenoma 1 pancreatic intraductal papillary-mucinous carcinoma hemorrhagic disease Pick disease pituitary gland adenoma congenital anemia invasive ductal breast carcinoma malignant ependymoma cystic fibrosis ACTH-dependent Cushing syndrome pseudohypoaldosteronism type 1 endogenous depression neurofibromatosis type 1 fibrous dysplasia Alzheimer disease glioblastoma osteosarcoma astrocytic tumor pancreatic intraductal papillary-mucinous neoplasm autosomal recessive pseudohypoaldosteronism type 1

Protein Modification Annotation

Modified Residue(s)
Chain	Residue(s)	Description
E, F, G, H	ACE	RESID: AA0041 , AA0042 , AA0043 , AA0044 , AA0045 , AA0046 , AA0049 , AA0050 , AA0051 , AA0052 , AA0053 , AA0054 , AA0354 PSI-MOD : N-acetyl-L-alanine MOD:00050 , N-acetyl-L-aspartic acid MOD:00051 , N-acetyl-L-cysteine MOD:00052 , N-acetyl-S-archeol-cysteine MOD:00897 , N-acetyl-L-glutamic acid MOD:00053 , N-acetyl-L-glutamine MOD:00054 , N-acetylglycine MOD:00055 , N-acetyl-L-methionine MOD:00058 , N-acetyl-L-proline MOD:00059 , N-acetyl-L-serine MOD:00060 , N,O-diacetylated L-serine MOD:00648 , N-acetyl-L-threonine MOD:00061 , N-acetyl-L-tyrosine MOD:00062 , N-acetyl-L-valine MOD:00063 , N2-acetyl-L-arginine MOD:00359
E, F, G, H	DTY	RESID: AA0041 , AA0042 , AA0043 , AA0044 , AA0045 , AA0046 , AA0049 , AA0050 , AA0051 , AA0052 , AA0053 , AA0054 , AA0354
E, F, G, H	NH2	RESID: AA0041 , AA0042 , AA0043 , AA0044 , AA0045 , AA0046 , AA0049 , AA0050 , AA0051 , AA0052 , AA0053 , AA0054 , AA0354 , AA0081 , AA0083 , AA0084 , AA0086 , AA0087 , AA0088 , AA0090 , AA0091 , AA0092 , AA0093 , AA0095 , AA0096 , AA0097 , AA0098 , AA0099 , AA0100 PSI-MOD : N-acetyl-L-alanine MOD:00050 , N-acetyl-L-aspartic acid MOD:00051 , N-acetyl-L-cysteine MOD:00052 , N-acetyl-S-archeol-cysteine MOD:00897 , N-acetyl-L-glutamic acid MOD:00053 , N-acetyl-L-glutamine MOD:00054 , N-acetylglycine MOD:00055 , N-acetyl-L-methionine MOD:00058 , N-acetyl-L-proline MOD:00059 , N-acetyl-L-serine MOD:00060 , N,O-diacetylated L-serine MOD:00648 , N-acetyl-L-threonine MOD:00061 , N-acetyl-L-tyrosine MOD:00062 , N-acetyl-L-valine MOD:00063 , N2-acetyl-L-arginine MOD:00359 , L-alanine amide MOD:00090 , L-asparagine amide MOD:00092 , L-aspartic acid 1-amide MOD:00093 , L-glutamine amide MOD:00095 , L-glutamic acid 1-amide MOD:00096 , glycine amide MOD:00097 , L-isoleucine amide MOD:00099 , L-leucine amide MOD:00100 , L-lysine amide MOD:00101 , L-methionine amide MOD:00102 , L-proline amide MOD:00104 , L-serine amide MOD:00105 , L-threonine amide MOD:00106 , L-tryptophan amide MOD:00107 , L-tyrosine amide MOD:00108 , L-valine amide MOD:00109

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.