Annotations: 7MJZ

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Radical_SAM,TRAM	e7mjzA1	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: Radical_SAM,TRAM	ECOD (1.6)
A	UPF0004	e7mjzA2	A: a/b three-layered sandwiches	X: Flavodoxin-like	H: Class I glutamine amidotransferase-like	T: CheY-like	F: UPF0004	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.40.50.12160	Alpha Beta	3-Layer(aba) Sandwich	Rossmann fold	Methylthiotransferase, N-terminal domain	CATH (utative)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF04055	Radical SAM superfamily (Radical_SAM)	Radical SAM superfamily	Radical SAM proteins catalyse diverse reactions, including unusual methylations, isomerisation, sulphur insertion, ring formation, anaerobic oxidation and protein radical formation.	Domain
A	PF01938	TRAM domain (TRAM)	TRAM domain	This small domain has no known function. However it may perform a nucleic acid binding role (Bateman A. unpublished observation).	Domain
A	PF00919	Uncharacterized protein family UPF0004 (UPF0004)	Uncharacterized protein family UPF0004	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase	methylthiotransferase activity alkylthioltransferase activity transferase activity, transferring sulphur-containing groups transferase activity catalytic activity iron-sulfur cluster binding binding 4 iron, 4 sulfur cluster binding metal cluster binding small molecule binding cation binding ion binding metal ion binding	tRNA methylthiolation tRNA modification RNA metabolic process primary metabolic process gene expression nucleobase-containing compound biosynthetic process macromolecule biosynthetic process nucleic acid metabolic process tRNA metabolic process nucleobase-containing compound metabolic process RNA biosynthetic process nucleic acid biosynthetic process RNA modification biosynthetic process tRNA processing tRNA methylthiolation tRNA modification RNA metabolic process primary metabolic process gene expression nucleobase-containing compound biosynthetic process macromolecule biosynthetic process nucleic acid metabolic process tRNA metabolic process nucleobase-containing compound metabolic process RNA biosynthetic process nucleic acid biosynthetic process RNA modification biosynthetic process tRNA processing macromolecule modification metabolic process cellular process macromolecule metabolic process RNA processing	cellular anatomical structure intracellular anatomical structure cytoplasm

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR007197	Radical SAM	Domain
A	IPR002792	TRAM domain	Domain
A	IPR006638	Elp3/MiaA/NifB-like, radical SAM core domain	Domain
A	IPR013848	Methylthiotransferase, N-terminal	Domain
A	IPR020612	Methylthiotransferase, conserved site	Conserved Site
A	IPR038135	Methylthiotransferase, N-terminal domain superfamily	Homologous Superfamily
A	IPR005839	Methylthiotransferase	Family
A	IPR023404	Radical SAM, alpha/beta horseshoe	Homologous Superfamily
A	IPR006463	tRNA-2-methylthio-N(6)-dimethylallyladenosine synthase MiaB	Family

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.