7NST | pdb_00007nst

ColicinE9 partial translocation complex

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Membrane Protein Annotation: OPM
Membrane Protein Annotation: PDBTM
Membrane Protein Annotation: MemProtMD
Membrane Protein Annotation: mpstruc

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Porins	8033399	3000224	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	Porins	8033399	3000224	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Porins	8033399	3000224	SCOP2B (2022-06-29)
E	SCOP2B Superfamily	YWTD domain-like	8042387	3000494	SCOP2B (2022-06-29)
E	SCOP2B Superfamily	TolB N-terminal domain-like	8042388	3000482	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Porin_1	e7nstA1	A: beta barrels	X: Outer membrane meander beta-barrels	H: Porins	T: Porin	F: Porin_1	ECOD (1.6)
C	Porin_1	e7nstC1	A: beta barrels	X: Outer membrane meander beta-barrels	H: Porins	T: Porin	F: Porin_1	ECOD (1.6)
B	Porin_1	e7nstB1	A: beta barrels	X: Outer membrane meander beta-barrels	H: Porins	T: Porin	F: Porin_1	ECOD (1.6)
E	DPPIV_N_1	e7nstE1	A: beta duplicates or obligate multimers	X: beta-propeller-like	H: beta-propeller	T: 6-bladed	F: DPPIV_N_1	ECOD (1.6)
E	TolB_N	e7nstE2	A: a/b three-layered sandwiches	X: TolB, N-terminal domain (From Topology)	H: TolB, N-terminal domain (From Topology)	T: TolB, N-terminal domain	F: TolB_N	ECOD (1.6)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C	PF00267	Gram-negative porin (Porin_1)	Gram-negative porin		Domain
E	PF07676	WD40-like Beta Propeller Repeat (PD40)	WD40-like Beta Propeller Repeat	-	Repeat
D	PF03515	Colicin-like bacteriocin tRNase domain (Cloacin)	Colicin-like bacteriocin tRNase domain	-	Family

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C	Outer membrane protein F	wide pore channel activity passive transmembrane transporter activity channel activity porin activity transporter activity transmembrane transporter activity binding lipid binding lipopolysaccharide binding carbohydrate derivative binding monoatomic ion channel activity monoatomic ion transmembrane transporter activity xenobiotic transmembrane transporter activity amide transmembrane transporter activity wide pore channel activity passive transmembrane transporter activity channel activity porin activity transporter activity transmembrane transporter activity binding lipid binding lipopolysaccharide binding carbohydrate derivative binding monoatomic ion channel activity monoatomic ion transmembrane transporter activity xenobiotic transmembrane transporter activity amide transmembrane transporter activity colicin transmembrane transporter activity bacteriocin transmembrane transporter activity disordered domain specific binding protein binding protein domain specific binding identical protein binding	localization establishment of protein localization nitrogen compound transport protein transport transport macromolecule localization intracellular protein localization establishment of localization transmembrane transport monoatomic ion transmembrane transport monoatomic ion transport cellular process protein-containing complex organization protein trimerization localization establishment of protein localization nitrogen compound transport protein transport transport macromolecule localization intracellular protein localization establishment of localization transmembrane transport monoatomic ion transmembrane transport monoatomic ion transport cellular process protein-containing complex organization protein trimerization cellular component organization cellular component biogenesis cellular component assembly protein homotrimerization protein complex oligomerization protein-containing complex assembly protein homooligomerization cellular component organization or biogenesis	cellular anatomical structure membrane protein-containing complex transmembrane transporter complex membrane protein complex transporter complex monoatomic ion channel complex pore complex cell periphery cell outer membrane external encapsulating structure cell envelope outer membrane
E	Tol-Pal system protein TolB	protein binding binding protein domain specific binding protein-containing complex binding	cell septum assembly cytokinesis cellular component biogenesis cellular component organization cellular component assembly cytokinetic process cellular process cell cycle cell division cellular component organization or biogenesis cell cycle process protein import localization establishment of protein localization cell septum assembly cytokinesis cellular component biogenesis cellular component organization cellular component assembly cytokinetic process cellular process cell cycle cell division cellular component organization or biogenesis cell cycle process protein import localization establishment of protein localization nitrogen compound transport protein transport transport macromolecule localization intracellular protein localization establishment of localization amide transport bacteriocin transport membrane organization regulation of biological process regulation of cellular component organization regulation of cellular process biological regulation membrane invagination regulation of membrane invagination cellular response to nitrogen compound cellular response to stimulus response to bacteriocin response to stimulus response to oxygen-containing compound response to nitrogen compound response to antibiotic cellular response to oxygen-containing compound cellular response to antibiotic cellular response to chemical stimulus cellular response to bacteriocin response to chemical	cellular anatomical structure membrane cell division site periplasmic space outer membrane-bounded periplasmic space cell envelope protein-containing complex
D	Colicin-E9	protein binding binding protein domain specific binding endonuclease activity nuclease activity catalytic activity, acting on a nucleic acid catalytic activity cation binding ion binding metal ion binding small molecule binding	biological process involved in interspecies interaction between organisms cellular process disruption of cell in another organism disruption of anatomical structure in another organism killing of cells of another organism cell killing defense response to bacterium response to stimulus response to other organism defense response response to bacterium response to stress response to external stimulus response to external biotic stimulus biological process involved in interspecies interaction between organisms cellular process disruption of cell in another organism disruption of anatomical structure in another organism killing of cells of another organism cell killing defense response to bacterium response to stimulus response to other organism defense response response to bacterium response to stress response to external stimulus response to external biotic stimulus response to biotic stimulus	intracellular anatomical structure cellular anatomical structure extrachromosomal circular DNA extrachromosomal DNA protein-containing complex

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C	IPR050298	Gram-negative bacterial outer membrane porin	Family
A, B, C	IPR001897	Porin, gammaproteobacterial	Family
A, B, C	IPR033900	Porin domain, Gram-negative type	Domain
A, B, C	IPR023614	Porin domain superfamily	Homologous Superfamily
A, B, C	IPR001702	Porin, Gram-negative type	Family
A, B, C	IPR013793	Porin, Gram-negative type, conserved site	Conserved Site
E	IPR011042	Six-bladed beta-propeller, TolB-like	Homologous Superfamily
E	IPR007195	TolB, N-terminal	Domain
E	IPR011659	WD40-like beta-propeller	Repeat
E	IPR014167	Tol-Pal system protein TolB	Family
D	IPR024575	Cloacin/colicin	Family
D	IPR016128	Pyosin/cloacin translocation domain	Domain
D	IPR044925	His-Me finger superfamily	Homologous Superfamily
D	IPR024566	Colicin, receptor domain	Domain
D	IPR036302	Pyosin/cloacin translocation domain superfamily	Homologous Superfamily
D	IPR037146	Colicin/pyocin, DNase domain superfamily	Homologous Superfamily

Membrane Protein Annotation: OPM OPM Database Homepage

Chains	External Link	Type	Class	Superfamily	Family
A, B, C, E	OPM	Transmembrane	Beta-barrel transmembrane	Trimeric porins (n=16,S=20)	General Bacterial Porin (GBP)

Membrane Protein Annotation: PDBTM PDBTM Database Homepage

Chains	External Link	Comment
A, B, C, E	PDBTM	Annotated as membrane protein by PDBTM

Membrane Protein Annotation: MemProtMD MemProtMD Database Homepage

Chains	External Link	Comment
A, B, C, E	MemProtMD	Annotated as membrane protein by MemProtMD

Membrane Protein Annotation: mpstruc mpstruc Database Homepage

Chains	Resource Link	Group	Subgroup	Protein
A, B, C, E	mpstruc	TRANSMEMBRANE PROTEINS: BETA-BARREL	Beta-Barrel Membrane Proteins: Porins and Relatives	OmpF and BtuB Porin in complex with colicin E9 (colE9)

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.