7PEE | pdb_00007pee

Crystal structure of extracellular part of human Trop2

External Resource: Annotation

Domain Annotation: ECOD Classification
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification
Pharos: Disease Associations
Protein Modification Annotation

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	EUF08377	e7peeA1	A: a+b two layers	X: Alpha-beta plaits	H: Ferredoxin-like domain of receptor-type protein tyrosine phosphatase (From Topology)	T: Ferredoxin-like domain of receptor-type protein tyrosine phosphatase	F: EUF08377	ECOD (1.6)
A	Thyroglobulin_1	e7peeA2	A: few secondary structure elements	X: Thyroglobulin type-1 domain (From Topology)	H: Thyroglobulin type-1 domain (From Topology)	T: Thyroglobulin type-1 domain	F: Thyroglobulin_1	ECOD (1.6)
A	F_UNCLASSIFIED	e7peeA3	A: few secondary structure elements	X: N-terminal domain of EpCAM (From Topology)	H: N-terminal domain of EpCAM (From Topology)	T: N-terminal domain of EpCAM	F: F_UNCLASSIFIED	ECOD (1.6)
B	EUF08377	e7peeB1	A: a+b two layers	X: Alpha-beta plaits	H: Ferredoxin-like domain of receptor-type protein tyrosine phosphatase (From Topology)	T: Ferredoxin-like domain of receptor-type protein tyrosine phosphatase	F: EUF08377	ECOD (1.6)
B	Thyroglobulin_1	e7peeB2	A: few secondary structure elements	X: Thyroglobulin type-1 domain (From Topology)	H: Thyroglobulin type-1 domain (From Topology)	T: Thyroglobulin type-1 domain	F: Thyroglobulin_1	ECOD (1.6)
B	F_UNCLASSIFIED	e7peeB3	A: few secondary structure elements	X: N-terminal domain of EpCAM (From Topology)	H: N-terminal domain of EpCAM (From Topology)	T: N-terminal domain of EpCAM	F: F_UNCLASSIFIED	ECOD (1.6)
C	EUF08377	e7peeC1	A: a+b two layers	X: Alpha-beta plaits	H: Ferredoxin-like domain of receptor-type protein tyrosine phosphatase (From Topology)	T: Ferredoxin-like domain of receptor-type protein tyrosine phosphatase	F: EUF08377	ECOD (1.6)
C	Thyroglobulin_1	e7peeC2	A: few secondary structure elements	X: Thyroglobulin type-1 domain (From Topology)	H: Thyroglobulin type-1 domain (From Topology)	T: Thyroglobulin type-1 domain	F: Thyroglobulin_1	ECOD (1.6)
C	F_UNCLASSIFIED	e7peeC3	A: few secondary structure elements	X: N-terminal domain of EpCAM (From Topology)	H: N-terminal domain of EpCAM (From Topology)	T: N-terminal domain of EpCAM	F: F_UNCLASSIFIED	ECOD (1.6)
D	EUF08377	e7peeD1	A: a+b two layers	X: Alpha-beta plaits	H: Ferredoxin-like domain of receptor-type protein tyrosine phosphatase (From Topology)	T: Ferredoxin-like domain of receptor-type protein tyrosine phosphatase	F: EUF08377	ECOD (1.6)
D	Thyroglobulin_1	e7peeD2	A: few secondary structure elements	X: Thyroglobulin type-1 domain (From Topology)	H: Thyroglobulin type-1 domain (From Topology)	T: Thyroglobulin type-1 domain	F: Thyroglobulin_1	ECOD (1.6)
D	F_UNCLASSIFIED	e7peeD3	A: few secondary structure elements	X: N-terminal domain of EpCAM (From Topology)	H: N-terminal domain of EpCAM (From Topology)	T: N-terminal domain of EpCAM	F: F_UNCLASSIFIED	ECOD (1.6)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D	PF21283	EPCAM/Trop-2, C-terminal (EPCAM-Trop-2_C)	EPCAM/Trop-2, C-terminal	EpCAM (epithelial cell adhesion molecule, also known as Trop-1) and Trop-2 (also known as tumor-associated calcium signal transducer 2, TACSTD2) are transmembrane glycoproteins that belong to the tumour-associated calcium signal transducer (TACSTD) f ...	EpCAM (epithelial cell adhesion molecule, also known as Trop-1) and Trop-2 (also known as tumor-associated calcium signal transducer 2, TACSTD2) are transmembrane glycoproteins that belong to the tumour-associated calcium signal transducer (TACSTD) family. They are evolutionarily related and both are implicated in signaling triggered by proteolytic cleavage within the ectodomain [1,3]. They share an overall similar structure but differ in their dimers formation. They consist of an ectodomain with an N-terminal, thyroglobulin type-1 (TY, Pfam:PF00086) and C-terminal domains. This entry represents the C-terminal domain of EpCAM and Trop-2, which has an alpha+beta fold [1-3].	Domain
A, B, C, D	PF00086	Thyroglobulin type-1 repeat (Thyroglobulin_1)	Thyroglobulin type-1 repeat	Thyroglobulin type 1 repeats are thought to be involved in the control of proteolytic degradation [2]. The domain usually contains six conserved cysteines. These form three disulphide bridges. Cysteines 1 pairs with 2, 3 with 4 and 5 with 6.	Domain
A, B, C, D	PF18635	Epithelial cell adhesion molecule, N-terminal domain (EpCAM_N)	Epithelial cell adhesion molecule, N-terminal domain	EpCAM (epithelial cell adhesion molecule), a stem and carcinoma cell marker, is a cell surface protein involved in homotypic cell-cell adhesion via intercellular oligomerization and proliferative signalling via proteolytic cleavage. Structure analysi ...	EpCAM (epithelial cell adhesion molecule), a stem and carcinoma cell marker, is a cell surface protein involved in homotypic cell-cell adhesion via intercellular oligomerization and proliferative signalling via proteolytic cleavage. Structure analysis indicate that it is composed of three domains: N-domain, Thyroglobulin type-1A (TY) domain and the C-terminal domain. This entry represents the small and compact disulphide-rich N-terminal domain of 39 amino-acid residues [1]. Trop-2 (also known as tumor-associated calcium signal transducer 2, TACSTD2) is also included in this entry, as they are evolutionarily related and both are implicated in signaling triggered by proteolytic cleavage within the ectodomain [1-3]. They share an overall similar structure but differ in their dimers formation.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D	Tumor-associated calcium signal transducer 2	-	multicellular organismal process negative regulation of locomotion regulation of cell motility regulation of cellular process negative regulation of biological process tissue migration negative regulation of epithelial cell migration ameboidal-type cell migration regulation of cell migration negative regulation of cellular process regulation of multicellular organismal process cell migration epithelial cell migration cell motility multicellular organismal process negative regulation of locomotion regulation of cell motility regulation of cellular process negative regulation of biological process tissue migration negative regulation of epithelial cell migration ameboidal-type cell migration regulation of cell migration negative regulation of cellular process regulation of multicellular organismal process cell migration epithelial cell migration cell motility regulation of epithelial cell migration locomotion negative regulation of cell migration regulation of biological process biological regulation epithelium migration regulation of locomotion negative regulation of cell motility cellular process negative regulation of multicellular organismal process developmental process cellular developmental process regulation of developmental process positive regulation of biological process regulation of cell differentiation positive regulation of developmental process positive regulation of stem cell differentiation stem cell differentiation positive regulation of cell differentiation cell differentiation regulation of stem cell differentiation positive regulation of cellular process cell population proliferation regulation of cell population proliferation regulation of epithelial cell proliferation epithelial cell proliferation mesonephric epithelium development kidney epithelium development nephron morphogenesis nephron tubule morphogenesis nephron epithelium morphogenesis nephron tubule development renal system development ureteric bud morphogenesis multicellular organism development tube morphogenesis anatomical structure development renal tubule development animal organ morphogenesis epithelial tube morphogenesis nephron development system development nephron epithelium development ureteric bud development epithelium development mesonephros development tube development animal organ development morphogenesis of an epithelium tissue development tissue morphogenesis anatomical structure morphogenesis kidney morphogenesis mesonephric tubule morphogenesis kidney development mesonephric tubule development renal tubule morphogenesis regulation of cytoskeleton organization regulation of cellular component organization regulation of actin filament-based process regulation of actin filament bundle assembly cellular component biogenesis cellular component organization cellular component assembly actin filament bundle organization actin filament-based process cellular component organization or biogenesis stress fiber assembly negative regulation of supramolecular fiber organization regulation of actin filament organization negative regulation of stress fiber assembly actin cytoskeleton organization actomyosin structure organization negative regulation of cellular component organization negative regulation of cytoskeleton organization actin filament organization regulation of cellular component biogenesis negative regulation of actin filament bundle assembly regulation of organelle organization regulation of stress fiber assembly organelle organization negative regulation of organelle organization contractile actin filament bundle assembly regulation of actomyosin structure organization regulation of actin cytoskeleton organization actin filament bundle assembly regulation of supramolecular fiber organization supramolecular fiber organization cytoskeleton organization sensory perception of light stimulus system process sensory perception nervous system process visual perception regulation of cell-substrate adhesion regulation of cell adhesion regulation of substrate adhesion-dependent cell spreading cell-substrate adhesion negative regulation of cell adhesion cell adhesion negative regulation of substrate adhesion-dependent cell spreading substrate adhesion-dependent cell spreading negative regulation of cell-substrate adhesion ruffle organization cell projection organization plasma membrane bounded cell projection assembly negative regulation of ruffle assembly ruffle assembly negative regulation of plasma membrane bounded cell projection assembly negative regulation of cell projection organization plasma membrane bounded cell projection organization regulation of cell projection organization regulation of cell projection assembly regulation of plasma membrane bounded cell projection assembly regulation of ruffle assembly cell projection assembly regulation of plasma membrane bounded cell projection organization branching morphogenesis of an epithelial tube regulation of kidney development regulation of branching involved in ureteric bud morphogenesis negative regulation of branching involved in ureteric bud morphogenesis regulation of morphogenesis of a branching structure regulation of animal organ morphogenesis branching involved in ureteric bud morphogenesis regulation of morphogenesis of an epithelium regulation of anatomical structure morphogenesis negative regulation of developmental process morphogenesis of a branching structure negative regulation of morphogenesis of an epithelium morphogenesis of a branching epithelium	cellular anatomical structure membrane cell periphery basal part of cell plasma membrane basal plasma membrane plasma membrane region extracellular exosome membrane-bounded organelle extracellular vesicle extracellular organelle extracellular region organelle extracellular space cellular anatomical structure membrane cell periphery basal part of cell plasma membrane basal plasma membrane plasma membrane region extracellular exosome membrane-bounded organelle extracellular vesicle extracellular organelle extracellular region organelle extracellular space vesicle extracellular membrane-bounded organelle intracellular anatomical structure cytoplasm cytosol lateral plasma membrane intracellular organelle nucleus intracellular membrane-bounded organelle

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D	IPR043406	Transmembrane glycoprotein EPCAM/Trop-2	Family
A, B, C, D	IPR049420	EPCAM/Trop-2, C-terminal	Domain
A, B, C, D	IPR041630	Epithelial cell adhesion molecule N-terminal domain	Domain
A, B, C, D	IPR036857	Thyroglobulin type-1 superfamily	Homologous Superfamily
A, B, C, D	IPR000716	Thyroglobulin type-1	Domain

Pharos: Disease Associations Pharos Homepage Annotation

Chains	Drug Target	Associated Disease
A, B, C, D	Pharos: P09758	gelatinous drop-like corneal dystrophy amyloidosis endometriosis pancreatic intraductal papillary-mucinous neoplasm Triple negative breast neoplasms corneal dystrophy ductal breast carcinoma in situ lung carcinoma nasopharyngeal carcinoma pancreatic ductal adenocarcinoma pulmonary hypertension tuberculosis acquired metabolic disease Crohn disease interstitial cystitis gelatinous drop-like corneal dystrophy amyloidosis endometriosis pancreatic intraductal papillary-mucinous neoplasm Triple negative breast neoplasms corneal dystrophy ductal breast carcinoma in situ lung carcinoma nasopharyngeal carcinoma pancreatic ductal adenocarcinoma pulmonary hypertension tuberculosis acquired metabolic disease Crohn disease interstitial cystitis malignant pancreatic neoplasm osteosarcoma pancreatic intraductal papillary-mucinous carcinoma spina bifida ulcerative colitis cystic fibrosis lung cancer MALT lymphoma ovarian cancer

Protein Modification Annotation

Modified Residue(s)
Chain	Residue(s)	Description
A, B, C, D	PCA	Parent Component: GLN RESID: AA0031 PSI-MOD : 2-pyrrolidone-5-carboxylic acid (Gln) MOD:00040 , 2-pyrrolidone-5-carboxylic acid (Glu) MOD:00420

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.