THE SOLUTION STRUCTURE OF A SITE-DIRECTED MUTANT (R111M) OF HUMAN CELLULAR RETIONIC ACID BINDING PROTEIN-TYPE II, NMR, 31 STRUCTURES
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | 2D HOMONUCLEAR | PBS | 7.3 | 300 | ||||
| 2 | 3D 15N-EDITED NMR EXPERIMENTS | PBS | 7.3 | 300 | ||||
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Varian | VXR500 | 500 |
| 2 | Bruker | DMX750 | 750 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| DGSA | THE STRUCTURES WERE CALCULATED FOLLOWING THE STANDARD DISTANCE GEOMETRY-SIMULATED ANNEALING PROTOCAL IN X-PLOR 3.1. SEVERAL ROUNDS OF REFINEMENT WERE PERFORMED. | X-PLOR |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | LEAST RESTRAINT VIOLATION AND LOWEST TOTAL ENERGY |
| Conformers Calculated Total Number | 75 |
| Conformers Submitted Total Number | 31 |
| Representative Model | 1 (n/a) |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | refinement | X-PLOR | 3.1 | BRUNGER |
| 2 | structure solution | X-PLOR | ||
