1C20 | pdb_00001c20

SOLUTION STRUCTURE OF THE DNA-BINDING DOMAIN FROM THE DEAD RINGER PROTEIN

SOLUTION NMR

NMR Experiment
Experiment	Type	Sample Contents	Solvent	Ionic Strength	pH	Pressure	Temperature (K)	Spectrometer
1	SEE PRIMARY REFERENCE	1.5MM DEAD RINGER U-15N; 20MM TRIS-HCL (PH 6.7); 100MM NACL; 1.5MM ZNCL2; 2MM DTT; 0.01% NAN3		0.10	6.7	AMBIENT	303

NMR Spectrometer Information
Spectrometer	Manufacturer	Model	Field Strength
1	Bruker	DRX	500
2	Bruker	DRX	600

NMR Refinement
Method	Details	Software
SEE PRIMARY REFERENCE		NMRPipe

NMR Ensemble Information
Conformer Selection Criteria	STRUCTURES WITH ACCEPTABLE COVALENT GEOMETRY, WITH FAVORABLE NON-BOND ENERGY, AND WITH THE LEAST RESTRAINT VIOLATIONS.
Conformers Calculated Total Number	100
Conformers Submitted Total Number	21
Representative Model	21 (minimized average structure)

Additional NMR Experimental Information
Details	SEE PRIMARY REFERENCE

Computation: NMR Software
#	Classification	Version	Software Name	Author
1	processing	NMRPipe	1999	DELAGLIO, F.
2	data analysis	NMRView	4.0.3	JOHNSON, B.
3	structure solution	X-PLOR	3.8	BRUNGER, A.
4	refinement	X-PLOR	3.8	BRUNGER, A.

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.