Solution Structure of RNA Polymerase Subunit RPB5 from Methanobacterium Thermoautotrophicum
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | 3D_13C-separated_NOESY | u-15n, 13c; 150 mM NaCl, 25 mM phosphate, 10% D2O, pH 6.5 | 90% H2O/10% D2O | 150 mM NaCl | 6.5 | 1 atm | 298 | |
| 2 | 3D_15N-separated_NOESY | u-15n, 13c; 150 mM NaCl, 25 mM phosphate, 10% D2O, pH 6.5 | 90% H2O/10% D2O | 150 mM NaCl | 6.5 | 1 atm | 298 | |
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Varian | INOVA | 600 |
| 2 | Varian | INOVA | 500 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| distance geometry simulated annealing | NMRPipe | |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | structures with the lowest energy |
| Conformers Calculated Total Number | 50 |
| Conformers Submitted Total Number | 10 |
| Additional NMR Experimental Information | |
|---|---|
| Details | The structure was determined using triple-resonance NMR spectroscopy |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | processing | NMRPipe | ||
| 2 | data analysis | NMRView | 3.0 | |
| 3 | refinement | X-PLOR | BRUNGER | |
