STRUCTURE OF THE DOUBLE-STRANDED RNA-BINDING DOMAIN OF THE PROTEIN KINASE PKR REVEALS THE MOLECULAR BASIS OF ITS DSRNA-MEDIATED ACTIVATION
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | 3D HNCACB | 6.5 | 298 | |||||
| 2 | 3D CBCA(CO)NH | 6.5 | 298 | |||||
| 3 | 3D H(CCO)NH | 6.5 | 298 | |||||
| 4 | 3D H(CCO)NH | 6.5 | 298 | |||||
| 5 | 3D HCCH-TOCSY | 6.5 | 298 | |||||
| 6 | 4D_15N | 6.5 | 298 | |||||
| 7 | 13C_EDITED_NOESY | 6.5 | 298 | |||||
| 8 | 4D_13C | 6.5 | 298 | |||||
| 9 | 2D_15N HOHAHA | 6.5 | 298 | |||||
| 10 | 3D_15N | 6.5 | 298 | |||||
| 11 | 3D HNHA | 6.5 | 298 | |||||
| 12 | 3D HNHB | 6.5 | 298 | |||||
| 13 | 2D-ARO-(HB)CB(CGCD)HD | 6.5 | 298 | |||||
| 14 | 2D-ARO_(HB)CB(CGCDCE)HE | 6.5 | 298 | |||||
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Varian | UNITY INOVA | 500 |
| 2 | Varian | UNITY INOVA | 600 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| MODIFIED DG/SA PROTOCOL IN XPLOR | X-PLOR | |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | |
| Conformers Calculated Total Number | 90 |
| Conformers Submitted Total Number | 21 |
| Representative Model | 21 (minimized average structure) |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | refinement | X-PLOR | BRUNGER, A. T. | |
| 2 | structure solution | NMRPipe | ||
| 3 | structure solution | PIPP | ||
