1VTG | pdb_00001vtg

THE MOLECULAR STRUCTURE OF A DNA-TRIOSTIN A COMPLEX

X-RAY DIFFRACTION

Crystallization

Crystalization Experiments
ID	Method	pH	Temperature	Details
1	VAPOR DIFFUSION	7		pH 7.00, VAPOR DIFFUSION

Crystal Properties
Matthews coefficient	Solvent content
4.14	70.28

Crystal Data

Unit Cell
Length ( Å )	Angle ( ˚ )
a = 31.35	α = 90
b = 62.38	β = 90
c = 61.26	γ = 90

Symmetry
Space Group	F 2 2 2

Diffraction

Diffraction Experiment
ID #	Crystal ID	Scattering Type	Data Collection Temperature	Detector	Detector Type	Details	Collection Date	Monochromator	Protocol
1	1	x-ray	257	DIFFRACTOMETER	NICOLET P3			M	SINGLE WAVELENGTH

Data Collection

Refinement

Statistics
Diffraction ID	Structure Solution Method	Resolution (High)	Resolution (Low)	Number Reflections (R-Free)	Percent Reflections (Observed)	R-Factor (Observed)	R-Work (Depositor)	R-Free (Depositor)	Mean Isotropic B
X-RAY DIFFRACTION		1.67				0.189

Temperature Factor Modeling
Anisotropic B[1][1]	Anisotropic B[1][2]	Anisotropic B[1][3]	Anisotropic B[2][2]	Anisotropic B[2][3]	Anisotropic B[3][3]

Non-Hydrogen Atoms Used in Refinement
Non-Hydrogen Atoms	Number
Protein Atoms	52
Nucleic Acid Atoms	120
Solvent Atoms
Heterogen Atoms	24

Software

Software
Software Name	Purpose
NUCLSQ	refinement

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.