1W9G | pdb_00001w9g

Structure of ERH (Enhencer of Rudimentary Gene)


X-RAY DIFFRACTION

Crystallization

Crystalization Experiments
IDMethodpHTemperatureDetails
17.510% ISOPROPANOL, 20% PEG4000, 0.1M HEPES (PH7.5), pH 7.50
Crystal Properties
Matthews coefficientSolvent content
2.551

Crystal Data

Unit Cell
Length ( Å )Angle ( ˚ )
a = 45.48α = 90
b = 62.676β = 117.96
c = 48.564γ = 90
Symmetry
Space GroupP 1 21 1

Diffraction

Diffraction Experiment
ID #Crystal IDScattering TypeData Collection TemperatureDetectorDetector TypeDetailsCollection DateMonochromatorProtocol
11x-ray100CCDMARRESEARCH2004-04-17MSINGLE WAVELENGTH
Radiation Source
ID #SourceTypeWavelength ListSynchrotron SiteBeamline
1SYNCHROTRONAPS BEAMLINE 22-IDAPS22-ID

Data Collection

Overall
ID #Resolution (High)Resolution (Low)Percent Possible (Observed)R Merge I (Observed)Net I Over Average Sigma (I)RedundancyNumber Reflections (All)Number Reflections (Observed)Observed Criterion Sigma (F)Observed Criterion Sigma (I)B (Isotropic) From Wilson Plot
1242.995.30.032.5155852.8
Highest Resolution Shell
ID #Resolution (High)Resolution (Low)Percent Possible (All)Percent Possible (Observed)Mean I Over Sigma (Observed)RedundancyNumber Unique Reflections (All)
122.1193.9

Refinement

Statistics
Diffraction IDStructure Solution MethodCross Validation methodResolution (High)Resolution (Low)Number Reflections (Observed)Number Reflections (R-Free)Percent Reflections (Observed)R-Factor (Observed)R-Work (Depositor)R-Free (Depositor)R-Free Selection DetailsMean Isotropic B
X-RAY DIFFRACTIONOTHERTHROUGHOUT243.031478676594.80.1990.1960.257RANDOM26.97
Temperature Factor Modeling
Anisotropic B[1][1]Anisotropic B[1][2]Anisotropic B[1][3]Anisotropic B[2][2]Anisotropic B[2][3]Anisotropic B[3][3]
-0.960.041.64-0.65
RMS Deviations
KeyRefinement Restraint Deviation
r_scangle_it7.151
r_dihedral_angle_1_deg6.906
r_scbond_it4.756
r_mcangle_it2.967
r_angle_refined_deg2.346
r_mcbond_it1.728
r_angle_other_deg1.144
r_symmetry_vdw_other0.345
r_symmetry_hbond_refined0.321
r_symmetry_vdw_refined0.308
RMS Deviations
KeyRefinement Restraint Deviation
r_scangle_it7.151
r_dihedral_angle_1_deg6.906
r_scbond_it4.756
r_mcangle_it2.967
r_angle_refined_deg2.346
r_mcbond_it1.728
r_angle_other_deg1.144
r_symmetry_vdw_other0.345
r_symmetry_hbond_refined0.321
r_symmetry_vdw_refined0.308
r_nbd_other0.26
r_nbd_refined0.229
r_xyhbond_nbd_refined0.219
r_chiral_restr0.17
r_nbtor_other0.099
r_bond_refined_d0.041
r_gen_planes_refined0.013
r_gen_planes_other0.008
r_bond_other_d0.002
r_dihedral_angle_2_deg
r_dihedral_angle_3_deg
r_dihedral_angle_4_deg
r_nbtor_refined
r_xyhbond_nbd_other
r_metal_ion_refined
r_metal_ion_other
r_symmetry_hbond_other
r_symmetry_metal_ion_refined
r_symmetry_metal_ion_other
r_mcbond_other
r_mcangle_other
r_scbond_other
r_scangle_other
r_long_range_B_refined
r_long_range_B_other
r_rigid_bond_restr
r_sphericity_free
r_sphericity_bonded
Non-Hydrogen Atoms Used in Refinement
Non-Hydrogen AtomsNumber
Protein Atoms1599
Nucleic Acid Atoms
Solvent Atoms104
Heterogen Atoms

Software

Software
Software NamePurpose
REFMACrefinement
MOSFLMdata reduction
MOSFLMdata scaling
SOLVEphasing