1XV0 | pdb_00001xv0

Solution NMR structure of RNA internal loop with three consecutive sheared GA pairs in 5'GGUGGAGGCU/3'PCCGAAGCCG

SOLUTION NMR

NMR Experiment
Experiment	Type	Sample Contents	Solvent	Ionic Strength	pH	Pressure	Temperature (K)	Spectrometer
1	2D TOCSY	80 mM NaCl, 10 mM Sodium phosphates, 0.5 mM EDTA, pH5.9 10% D2O	NMR		5.9	1 atm	303
2	2D NOESY	80 mM NaCl, 10 mM Sodium phosphates, 0.5 mM EDTA, pH5.9 10% D2O	NMR		5.9	1 atm	303
3	DQF-COSY	80 mM NaCl, 10 mM Sodium phosphates, 0.5 mM EDTA, pH5.9 10% D2O	NMR		5.9	1 atm	273
4	2D NOESY	80 mM NaCl, 10 mM Sodium phosphates, 0.5 mM EDTA, pH5.9 10% D2O	NMR		5.9	1 atm	273

NMR Spectrometer Information
Spectrometer	Manufacturer	Model	Field Strength
1	Varian	INOVA	500
2	Varian	INOVA	600

NMR Ensemble Information
Conformer Selection Criteria	structures with the least restraint violations
Conformers Calculated Total Number	40
Conformers Submitted Total Number	33
Representative Model	17 (closest to the average)

Computation: NMR Software
#	Classification	Version	Software Name	Author
1	data analysis	Felix	2000
2	structure solution	Discover
3	refinement	Discover
4	structure solution	Amber

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.