2LOQ | pdb_00002loq

Backbone structure of human membrane protein FAM14B (Interferon alpha-inducible protein 27-like protein 1)


SOLUTION NMR
NMR Experiment
ExperimentTypeSample ContentsSolventIonic StrengthpHPressureTemperature (K)Spectrometer
12D 1H-15N HSQCselectively labeled 13C(1),15N-FAM14B, 20 mM MES-Bis-TRIS, 3 % LMPG, 0.5 mM DSS95% H2O/5% D2O606.0ambient310
22D HNCOselectively labeled 13C(1),15N-FAM14B, 20 mM MES-Bis-TRIS, 3 % LMPG, 0.5 mM DSS95% H2O/5% D2O606.0ambient310
33D HNCO13C-15N-FAM14B, 20 mM MES-Bis-TRIS, 3 % LMPG, 0.5 mM DSS95% H2O/5% D2O606.0ambient310
43D HNCA13C-15N-FAM14B, 20 mM MES-Bis-TRIS, 3 % LMPG, 0.5 mM DSS95% H2O/5% D2O606.0ambient310
53D HNCACB13C-15N-FAM14B, 20 mM MES-Bis-TRIS, 3 % LMPG, 0.5 mM DSS95% H2O/5% D2O606.0ambient310
62D 1H-15N HSQC15N-FAM14B, 20 mM MES-Bis-TRIS, 3 % LMPG, 0.5 mM DSS95% H2O/5% D2O606.0ambient310
73D 1H-15N NOESY15N-FAM14B, 20 mM MES-Bis-TRIS, 3 % LMPG, 0.5 mM DSS95% H2O/5% D2O606.0ambient310
83D 1H-15N NOESY15N,2H-FAM14B, 20 mM MES-Bis-TRIS, 3 % LMPG, 0.5 mM DSS95% H2O/5% D2O606.0ambient310
93D 13C-15N HSQC-NOESY-HSQC13C-15N-FAM14B, 20 mM MES-Bis-TRIS, 3 % LMPG, 0.5 mM DSS95% H2O/5% D2O606.0ambient310
NMR Spectrometer Information
SpectrometerManufacturerModelField Strength
1BrukerDRX700
NMR Refinement
MethodDetailsSoftware
torsion angle dynamicsTopSpin
NMR Ensemble Information
Conformer Selection Criteriatarget function
Conformers Calculated Total Number200
Conformers Submitted Total Number20
Representative Model1 (fewest violations)
Computation: NMR Software
#ClassificationVersionSoftware NameAuthor
1collectionTopSpinBruker Biospin
2processingTopSpinBruker Biospin
3processingPROSAGuntert
4chemical shift assignmentCARAKeller and Wuthrich
5data analysisCARAKeller and Wuthrich
6structure solutionCYANAGuntert, Mumenthaler and Wuthrich
7structure visualizationMOLMOLKoradi, Billeter and Wuthrich
8structure analysisMOLMOLKoradi, Billeter and Wuthrich
9refinementCYANAGuntert, Mumenthaler and Wuthrich