ELECTRON MICROSCOPY
Starting Model(s)
| Initial Refinement Model(s) | |||
|---|---|---|---|
| Type | Source | Accession Code | Details |
| experimental model | PDB | 2MS2 | |
| Sample |
|---|
| MS2 BOUND TO F-PILUS |
| Specimen Preparation | |
|---|---|
| Sample Aggregation State | PARTICLE |
| Vitrification Instrument | HOMEMADE PLUNGER |
| Cryogen Name | ETHANE |
| Sample Vitrification Details | LIQUID ETHANE HOME MADE FREEZING DEVICE |
| 3D Reconstruction | |
|---|---|
| Reconstruction Method | SINGLE PARTICLE |
| Number of Particles | 1500 |
| Reported Resolution (Å) | 39 |
| Resolution Method | |
| Other Details | THREE COORDINATE FILES (4BP4, 4BP5, 4BP6) LINKED TO 1 EM STRUCTURE EMD-2365. |
| Refinement Type | |
| Symmetry Type | POINT |
| Point Symmetry | C1 |
| Map-Model Fitting and Refinement | |||||
|---|---|---|---|---|---|
| Id | 1 (2MS2) | ||||
| Refinement Space | REAL | ||||
| Refinement Protocol | RIGID BODY FIT | ||||
| Refinement Target | |||||
| Overall B Value | |||||
| Fitting Procedure | |||||
| Details | METHOD--RIGID BODY REFINEMENT PROTOCOL--RIGID BODY | ||||
| Data Acquisition | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detector Type | GATAN ULTRASCAN 10000 (10k x 10k) | ||||||||
| Electron Dose (electrons/Å**2) | |||||||||
| Imaging Experiment | 1 |
|---|---|
| Date of Experiment | 2012-01-01 |
| Temperature (Kelvin) | |
| Microscope Model | FEI TECNAI 12 |
| Minimum Defocus (nm) | 3000 |
| Maximum Defocus (nm) | 3000 |
| Minimum Tilt Angle (degrees) | -60 |
| Maximum Tilt Angle (degrees) | 60 |
| Nominal CS | |
| Imaging Mode | BRIGHT FIELD |
| Specimen Holder Model | |
| Nominal Magnification | 23000 |
| Calibrated Magnification | 23000 |
| Source | LAB6 |
| Acceleration Voltage (kV) | 120 |
| Imaging Details |
| EM Software | ||
|---|---|---|
| Task | Software Package | Version |
| RECONSTRUCTION | IMOD | |
| RECONSTRUCTION | PEET | |
