4V3P | pdb_00004v3p

The molecular structure of the left-handed supra-molecular helix of eukaryotic polyribosomes


ELECTRON MICROSCOPY

Starting Model(s)

Initial Refinement Model(s)
TypeSourceAccession CodeDetails
experimental modelPDB 3IZ6 
Sample
POLY-RIBOSOME FROM IN VITRO WHEAT GERM SYSTEM
Specimen Preparation
Sample Aggregation StatePARTICLE
Vitrification InstrumentFEI VITROBOT MARK IV
Cryogen NameETHANE
Sample Vitrification DetailsVITRIFICATION 1 --CRYOGEN- ETHANE, HUMIDITY-95, TEMPERATURE- 120,INSTRUMENT- FEI VITROBOT MARK IV, METHOD- 3UL OF SAMPLE APPLIED ON 300 MESH HOLY CARB ...VITRIFICATION 1 --CRYOGEN- ETHANE, HUMIDITY-95, TEMPERATURE- 120,INSTRUMENT- FEI VITROBOT MARK IV, METHOD- 3UL OF SAMPLE APPLIED ON 300 MESH HOLY CARBON QUANTIFOIL GRID. BLOTTING WAS DONE IN VITROBOT MARK IV, BLOT TIME 0.5 SEC, BLOT FORCE 5
3D Reconstruction
Reconstruction MethodSUBTOMOGRAM AVERAGING
Number of Particles106
Reported Resolution (Å)34
Resolution Method
Other DetailsSUBMISSION BASED ON EXPERIMENTAL DATA
Refinement Type
Symmetry TypeHELICAL
Map-Model Fitting and Refinement
Id1 (3IZ6)
Refinement SpaceREAL
Refinement ProtocolRIGID BODY FIT
Refinement Target
Overall B Value
Fitting Procedure
DetailsMETHOD--RIGID BODY REFINEMENT PROTOCOL--X-RAY
Data Acquisition
Detector TypeFEI FALCON I (4k x 4k)
Electron Dose (electrons/Å**2)30
Imaging Experiment1
Date of Experiment2013-01-01
Temperature (Kelvin)90
Microscope ModelFEI TECNAI F30
Minimum Defocus (nm)2000
Maximum Defocus (nm)4000
Minimum Tilt Angle (degrees)-70
Maximum Tilt Angle (degrees)70
Nominal CS2
Imaging ModeBRIGHT FIELD
Specimen Holder Model
Nominal Magnification39000
Calibrated Magnification41176
SourceFIELD EMISSION GUN
Acceleration Voltage (kV)150
Imaging Details
EM Software
TaskSoftware PackageVersion
MODEL FITTINGUCSF Chimera
RECONSTRUCTIONIMAGIC
RECONSTRUCTIONIMOD
RECONSTRUCTIONXmipp