ELECTRON MICROSCOPY
| Sample |
|---|
| BOVINE POL II ELONGATION COMPLEX |
| Specimen Preparation | |
|---|---|
| Sample Aggregation State | PARTICLE |
| Vitrification Instrument | FEI VITROBOT MARK IV |
| Cryogen Name | ETHANE |
| Sample Vitrification Details | |
| 3D Reconstruction | |
|---|---|
| Reconstruction Method | SINGLE PARTICLE |
| Number of Particles | 264134 |
| Reported Resolution (Å) | 3.4 |
| Resolution Method | |
| Other Details | THE FOLLOWING REGIONS WERE MODELED INTO THE UNSHARPENED EC1 MAP (EMDB-3218) DUE TO WEAKER DENSITY. RPB1 433-437, RPB1 1103-1108 (POLY-ALANINE), RPB1 1 ... |
| Refinement Type | |
| Symmetry Type | POINT |
| Point Symmetry | C1 |
| Map-Model Fitting and Refinement | |||||
|---|---|---|---|---|---|
| Id | 1 | ||||
| Refinement Space | REAL | ||||
| Refinement Protocol | OTHER | ||||
| Refinement Target | |||||
| Overall B Value | 137 | ||||
| Fitting Procedure | |||||
| Details | REFINEMENT PROTOCOL--CRYO-EM | ||||
| Data Acquisition | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detector Type | GATAN K2 SUMMIT (4k x 4k) | ||||||||
| Electron Dose (electrons/Å**2) | 43 | ||||||||
| Imaging Experiment | 1 |
|---|---|
| Date of Experiment | 2014-12-01 |
| Temperature (Kelvin) | |
| Microscope Model | FEI TITAN KRIOS |
| Minimum Defocus (nm) | 600 |
| Maximum Defocus (nm) | 3100 |
| Minimum Tilt Angle (degrees) | |
| Maximum Tilt Angle (degrees) | |
| Nominal CS | 2 |
| Imaging Mode | BRIGHT FIELD |
| Specimen Holder Model | |
| Nominal Magnification | 37000 |
| Calibrated Magnification | 37037 |
| Source | FIELD EMISSION GUN |
| Acceleration Voltage (kV) | 300 |
| Imaging Details |
| EM Software | ||
|---|---|---|
| Task | Software Package | Version |
| RECONSTRUCTION | RELION | 1.3 |
| Image Processing | ||||
|---|---|---|---|---|
| CTF Correction Type | CTF Correction Details | Number of Particles Selected | Particle Selection Details | |
| INDIVIDUAL PARTICLES | ||||
