Download MendeleyNADH-bound AIF activates the mitochondrial CHCHD4/MIA40 chaperone by a substrate-mimicry mechanism
Brosey, C.A., Shen, R., Tainer, J.A.(2024) EMBO J
8VGY
Crystal structure of human apoptosis-inducing factor (AIF) bound to the fused N-terminal domain of CHCHD4
- PDB DOI: https://doi.org/10.2210/pdb8VGY/pdb
- Classification: OXIDOREDUCTASE
- Organism(s): Homo sapiens
- Expression System: Escherichia coli BL21(DE3), Escherichia coli
- Mutation(s): Yes
- Deposited: 2023-12-29 Released: 2025-01-08
- Funding Organization(s): Cancer Prevention and Research Institute of Texas (CPRIT), National Institutes of Health/National Cancer Institute (NIH/NCI), Other private
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 2.30 Å
- R-Value Free: 0.212
- R-Value Work: 0.181
- R-Value Observed: 0.182
Starting Model: experimental
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This is version 1.0 of the entry. See complete history.
Literature
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Apoptosis-inducing factor 1, mitochondrial,Mitochondrial intermembrane space import and assembly protein 40 | A, B [auth C] | 571 | Homo sapiens | Mutation(s): 1 Gene Names: AIFM1, AIF, PDCD8, CHCHD4, MIA40 EC: 1.6.99 |  |
UniProt & NIH Common Fund Data Resources | |||||
Find proteins for O95831 (Homo sapiens) Explore O95831 Go to UniProtKB: O95831 | |||||
PHAROS: O95831 GTEx: ENSG00000156709 | |||||
Find proteins for Q8N4Q1 (Homo sapiens) Explore Q8N4Q1 Go to UniProtKB: Q8N4Q1 | |||||
PHAROS: Q8N4Q1 GTEx: ENSG00000163528 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Groups | O95831Q8N4Q1 | ||||
Sequence AnnotationsExpand | |||||
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Small Molecules
| Ligands 2 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| FAD (Subject of Investigation/LOI) Query on FAD | C [auth A], F [auth C] | FLAVIN-ADENINE DINUCLEOTIDE C27 H33 N9 O15 P2 VWWQXMAJTJZDQX-UYBVJOGSSA-N |  | ||
| EDO Query on EDO | D [auth A], E [auth A] | 1,2-ETHANEDIOL C2 H6 O2 LYCAIKOWRPUZTN-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 2.30 Å
- R-Value Free: 0.212
- R-Value Work: 0.181
- R-Value Observed: 0.182
- Space Group: P 21 21 21
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 68.608 | α = 90 |
| b = 109.197 | β = 90 |
| c = 174.632 | γ = 90 |
| Software Name | Purpose |
|---|---|
| XDS | data reduction |
| Aimless | data scaling |
| PHASER | phasing |
| PHENIX | refinement |
Entry History & Funding Information
Deposition Data
- Released Date: 2025-01-08 Deposition Author(s): Brosey, C.A., Tainer, J.A.
| Funding Organization | Location | Grant Number |
|---|---|---|
| Cancer Prevention and Research Institute of Texas (CPRIT) | United States | RP180813 |
| National Institutes of Health/National Cancer Institute (NIH/NCI) | United States | R35 CA220430 |
| Other private | Robert A. Welch Chemistry Chair |
Revision History (Full details and data files)
- Version 1.0: 2025-01-08
Type: Initial release
