1JYH | pdb_00001jyh

Crystal Structure of the Escherichia coli SbmC protein (AKA Gyrase Inhibitory Protein GyrI, AKA YeeB)

PDB DOI: https://doi.org/10.2210/pdb1JYH/pdb

Classification: UNKNOWN FUNCTION
Organism(s): Escherichia coli
Expression System: Escherichia coli BL21
Mutation(s): No

Deposited: 2001-09-12 Released: 2002-04-10
Deposition Author(s): Romanowski, M.J., Gibney, S.A., Burley, S.K., New York SGX Research Center for Structural Genomics (NYSGXRC)

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 1.80 Å
R-Value Free:
0.222 (Depositor), 0.220 (DCC)
R-Value Work:
0.196 (Depositor), 0.200 (DCC)
R-Value Observed:
0.196 (Depositor)

wwPDB Validation 3D Report Full Report

This is version 1.4 of the entry. See complete history.

Literature

Crystal structure of the Escherichia coli SbmC protein that protects cells from the DNA replication inhibitor microcin B17.

Romanowski, M.J., Gibney, S.A., Burley, S.K.

(2002) Proteins 47: 403-407

PubMed: 11948793 Search on PubMed
DOI: https://doi.org/10.1002/prot.10102
Primary Citation of Related Structures:
1JYH

Organizational Affiliation

Laboratories of Molecular Biophysics, The Rockefeller University, New York, New York 10021, USA.

Macromolecule Content

Total Structure Weight: 18.1 kDa
Atom Count: 1,433
Modelled Residue Count: 155
Deposited Residue Count: 157
Unique protein chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
DNA Gyrase inhibitory protein	A	157	Escherichia coli	Mutation(s): 0 Gene Names: gyrI (sbmC, yeeB)
UniProt
Find proteins for P33012 (Escherichia coli (strain K12)) Explore P33012 Go to UniProtKB: P33012
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	P33012
Sequence Annotations Expand
Reference Sequence LOADING

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 1.80 Å
R-Value Free: 0.222 (Depositor), 0.220 (DCC)
R-Value Work: 0.196 (Depositor), 0.200 (DCC)
R-Value Observed: 0.196 (Depositor)

Space Group: P 4₃ 2₁ 2

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 72.44	α = 90
b = 72.44	β = 90
c = 80.891	γ = 90

Software Package:

Software Name	Purpose
MLPHARE	phasing
CNS	refinement
DENZO	data reduction
SCALEPACK	data scaling

Structure Validation

View Full Validation Report

Entry History

Deposition Data

Released Date: 2002-04-10

Deposition Author(s):

Romanowski, M.J.

New York SGX Research Center for Structural Genomics (NYSGXRC)

Revision History (Full details and data files)

Version 1.0: 2002-04-10
Type: Initial release
Version 1.1: 2008-04-27
Changes: Version format compliance
Version 1.2: 2011-07-13
Changes: Version format compliance
Version 1.3: 2021-02-03
Changes: Database references, Structure summary
Version 1.4: 2024-02-07
Changes: Data collection, Database references

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.