1HWT | pdb_00001hwt

STRUCTURE OF A HAP1/DNA COMPLEX REVEALS DRAMATICALLY ASYMMETRIC DNA BINDING BY A HOMODIMERIC PROTEIN

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
E [auth C]	d1hwtc1	Small proteins	Zn2/Cys6 DNA-binding domain	Zn2/Cys6 DNA-binding domain	Zn2/Cys6 DNA-binding domain	Hap1 (Cyp1)	baker's yeast (Saccharomyces cerevisiae ) [TaxId: 4932 ],	SCOPe (2.08)
E [auth C]	d1hwtc2	Coiled coil proteins	Parallel coiled-coil	Leucine zipper domain	Leucine zipper domain	HAP1	baker's yeast (Saccharomyces cerevisiae ) [TaxId: 4932 ],	SCOPe (2.08)
F [auth D]	d1hwtd1	Small proteins	Zn2/Cys6 DNA-binding domain	Zn2/Cys6 DNA-binding domain	Zn2/Cys6 DNA-binding domain	Hap1 (Cyp1)	baker's yeast (Saccharomyces cerevisiae ) [TaxId: 4932 ],	SCOPe (2.08)
F [auth D]	d1hwtd2	Coiled coil proteins	Parallel coiled-coil	Leucine zipper domain	Leucine zipper domain	HAP1	baker's yeast (Saccharomyces cerevisiae ) [TaxId: 4932 ],	SCOPe (2.08)
G	d1hwtg1	Small proteins	Zn2/Cys6 DNA-binding domain	Zn2/Cys6 DNA-binding domain	Zn2/Cys6 DNA-binding domain	Hap1 (Cyp1)	baker's yeast (Saccharomyces cerevisiae ) [TaxId: 4932 ],	SCOPe (2.08)
G	d1hwtg2	Coiled coil proteins	Parallel coiled-coil	Leucine zipper domain	Leucine zipper domain	HAP1	baker's yeast (Saccharomyces cerevisiae ) [TaxId: 4932 ],	SCOPe (2.08)
H	d1hwth1	Small proteins	Zn2/Cys6 DNA-binding domain	Zn2/Cys6 DNA-binding domain	Zn2/Cys6 DNA-binding domain	Hap1 (Cyp1)	baker's yeast (Saccharomyces cerevisiae ) [TaxId: 4932 ],	SCOPe (2.08)
H	d1hwth2	Coiled coil proteins	Parallel coiled-coil	Leucine zipper domain	Leucine zipper domain	HAP1	baker's yeast (Saccharomyces cerevisiae ) [TaxId: 4932 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
E [auth C]	SCOP2B Superfamily	Leucine zipper-like	8039474	3001609	SCOP2B (2022-06-29)
E [auth C]	SCOP2B Superfamily	Zn2/Cys6 DNA-binding domain	8039021	3001104	SCOP2B (2022-06-29)
F [auth D]	SCOP2B Superfamily	Zn2/Cys6 DNA-binding domain	8039021	3001104	SCOP2B (2022-06-29)
F [auth D]	SCOP2B Superfamily	Leucine zipper-like	8039474	3001609	SCOP2B (2022-06-29)
G	SCOP2B Superfamily	Zn2/Cys6 DNA-binding domain	8039021	3001104	SCOP2B (2022-06-29)
G	SCOP2B Superfamily	Leucine zipper-like	8039474	3001609	SCOP2B (2022-06-29)
H	SCOP2B Superfamily	Zn2/Cys6 DNA-binding domain	8039021	3001104	SCOP2B (2022-06-29)
H	SCOP2B Superfamily	Leucine zipper-like	8039474	3001609	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
E [auth C]	Zn_clus	e1hwtC1	A: few secondary structure elements	X: Zn2/Cys6 DNA-binding domain (From Topology)	H: Zn2/Cys6 DNA-binding domain (From Topology)	T: Zn2/Cys6 DNA-binding domain	F: Zn_clus	ECOD (1.6)
F [auth D]	Zn_clus	e1hwtD1	A: few secondary structure elements	X: Zn2/Cys6 DNA-binding domain (From Topology)	H: Zn2/Cys6 DNA-binding domain (From Topology)	T: Zn2/Cys6 DNA-binding domain	F: Zn_clus	ECOD (1.6)
G	Zn_clus	e1hwtG1	A: few secondary structure elements	X: Zn2/Cys6 DNA-binding domain (From Topology)	H: Zn2/Cys6 DNA-binding domain (From Topology)	T: Zn2/Cys6 DNA-binding domain	F: Zn_clus	ECOD (1.6)
H	Zn_clus	e1hwtH1	A: few secondary structure elements	X: Zn2/Cys6 DNA-binding domain (From Topology)	H: Zn2/Cys6 DNA-binding domain (From Topology)	T: Zn2/Cys6 DNA-binding domain	F: Zn_clus	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
E [auth C]	4.10.240.10	Few Secondary Structures	Irregular	CD2-Gal4	Zn(2)-C6 fungal-type DNA-binding domain	CATH (4.3.0)
E [auth C]	1.20.5.170	Mainly Alpha	Up-down Bundle	Single alpha-helices involved in coiled-coils or other helix-helix interfaces		CATH (4.3.0)
F [auth D]	4.10.240.10	Few Secondary Structures	Irregular	CD2-Gal4	Zn(2)-C6 fungal-type DNA-binding domain	CATH (4.3.0)
F [auth D]	1.20.5.170	Mainly Alpha	Up-down Bundle	Single alpha-helices involved in coiled-coils or other helix-helix interfaces		CATH (4.3.0)
G	4.10.240.10	Few Secondary Structures	Irregular	CD2-Gal4	Zn(2)-C6 fungal-type DNA-binding domain	CATH (4.3.0)
G	1.20.5.170	Mainly Alpha	Up-down Bundle	Single alpha-helices involved in coiled-coils or other helix-helix interfaces		CATH (4.3.0)
H	4.10.240.10	Few Secondary Structures	Irregular	CD2-Gal4	Zn(2)-C6 fungal-type DNA-binding domain	CATH (4.3.0)
H	1.20.5.170	Mainly Alpha	Up-down Bundle	Single alpha-helices involved in coiled-coils or other helix-helix interfaces		CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
E [auth C], F [auth D], G, H	PF00172	Fungal Zn(2)-Cys(6) binuclear cluster domain (Zn_clus)	Fungal Zn(2)-Cys(6) binuclear cluster domain		Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
E [auth C], F [auth D], G, H	PROTEIN (HEME ACTIVATOR PROTEIN)	DNA binding sequence-specific DNA binding binding double-stranded DNA binding nucleic acid binding RNA polymerase II transcription regulatory region sequence-specific DNA binding cis-regulatory region sequence-specific DNA binding sequence-specific double-stranded DNA binding transcription cis-regulatory region binding transcription regulatory region nucleic acid binding RNA polymerase II cis-regulatory region sequence-specific DNA binding cation binding transition metal ion binding zinc ion binding DNA binding sequence-specific DNA binding binding double-stranded DNA binding nucleic acid binding RNA polymerase II transcription regulatory region sequence-specific DNA binding cis-regulatory region sequence-specific DNA binding sequence-specific double-stranded DNA binding transcription cis-regulatory region binding transcription regulatory region nucleic acid binding RNA polymerase II cis-regulatory region sequence-specific DNA binding cation binding transition metal ion binding zinc ion binding ion binding metal ion binding small molecule binding DNA-binding transcription factor activity, RNA polymerase II-specific transcription regulator activity DNA-binding transcription factor activity DNA-binding transcription activator activity, RNA polymerase II-specific DNA-binding transcription activator activity	RNA metabolic process primary metabolic process nucleobase-containing compound biosynthetic process gene expression macromolecule biosynthetic process nucleic acid metabolic process RNA biosynthetic process nucleobase-containing compound metabolic process DNA-templated transcription nucleic acid biosynthetic process biosynthetic process metabolic process cellular process macromolecule metabolic process	membrane-bounded organelle intracellular organelle cellular anatomical structure intracellular anatomical structure organelle nucleus intracellular membrane-bounded organelle
B, D [auth F]	DNA (5'-D(GPCPTPAPAPTPAPGPCPGPAPTPAPAPTPAPGPCPGPC)-3')	-	-	-
A, C [auth E]	DNA (5'-D(GPCPGPCPTPAPTPTPAPTPCPGPCPTPAPTPTPAPGPC)-3')	-	-	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
E [auth C], F [auth D], G, H	IPR007219	Xylanolytic transcriptional activator, regulatory domain	Domain
E [auth C], F [auth D], G, H	IPR046347	Basic-leucine zipper domain superfamily	Homologous Superfamily
E [auth C], F [auth D], G, H	IPR051430	Fungal Transcription Factors for Environmental Response	Family
E [auth C], F [auth D], G, H	IPR036864	Zn(2)-C6 fungal-type DNA-binding domain superfamily	Homologous Superfamily
E [auth C], F [auth D], G, H	IPR001138	Zn(2)Cys(6) fungal-type DNA-binding domain	Domain

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.