1MIW | pdb_00001miw

Crystal structure of Bacillus stearothermophilus CCA-adding enzyme in complex with ATP

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d1miwa1	All alpha proteins	Poly A polymerase C-terminal region-like	Poly A polymerase C-terminal region-like	Poly A polymerase C-terminal region-like	tRNA CCA-adding enzyme, C-terminal domains	(Geobacillus stearothermophilus ) [TaxId: 1422 ],	SCOPe (2.08)
A	d1miwa2	Alpha and beta proteins (a+b)	Nucleotidyltransferase	Nucleotidyltransferase	Poly A polymerase head domain-like	tRNA CCA-adding enzyme, head domain	(Geobacillus stearothermophilus ) [TaxId: 1422 ],	SCOPe (2.08)
B	d1miwb1	All alpha proteins	Poly A polymerase C-terminal region-like	Poly A polymerase C-terminal region-like	Poly A polymerase C-terminal region-like	tRNA CCA-adding enzyme, C-terminal domains	(Geobacillus stearothermophilus ) [TaxId: 1422 ],	SCOPe (2.08)
B	d1miwb2	Alpha and beta proteins (a+b)	Nucleotidyltransferase	Nucleotidyltransferase	Poly A polymerase head domain-like	tRNA CCA-adding enzyme, head domain	(Geobacillus stearothermophilus ) [TaxId: 1422 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2 Family	Poly A polymerase C-terminal region-like	8031212	4002497	SCOP2 (2022-06-29)
A	SCOP2 Family	Poly A polymerase head domain-like	8031214	4003222	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	Poly A polymerase C-terminal region-like	8043590	3001334	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	Nucleotidyltransferase-like	8043592	3000129	SCOP2 (2022-06-29)
B	SCOP2B Superfamily	Poly A polymerase C-terminal region-like	8043590	3001334	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Nucleotidyltransferase-like	8043592	3000129	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	PolyA_pol_RNAbd	e1miwA1	A: alpha complex topology	X: PDEase-like	H: Poly A polymerase C-terminal region-like (From Topology)	T: Poly A polymerase C-terminal region-like	F: PolyA_pol_RNAbd	ECOD (1.6)
A	PolyA_pol	e1miwA2	A: a+b three layers	X: Nucleotidyltransferase-like	H: Nucleotidyltransferase (From Topology)	T: Nucleotidyltransferase	F: PolyA_pol	ECOD (1.6)
B	PolyA_pol_RNAbd	e1miwB1	A: alpha complex topology	X: PDEase-like	H: Poly A polymerase C-terminal region-like (From Topology)	T: Poly A polymerase C-terminal region-like	F: PolyA_pol_RNAbd	ECOD (1.6)
B	PolyA_pol	e1miwB2	A: a+b three layers	X: Nucleotidyltransferase-like	H: Nucleotidyltransferase (From Topology)	T: Nucleotidyltransferase	F: PolyA_pol	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.30.460.10	Alpha Beta	2-Layer Sandwich	Beta Polymerase	domain 2	CATH (4.3.0)
A	1.10.110.30	Mainly Alpha	Orthogonal Bundle	Hydrophobic Seed Protein		CATH (4.3.0)
A	1.20.58.560	Mainly Alpha	Up-down Bundle	Methane Monooxygenase Hydroxylase	Chain G, domain 1	CATH (4.3.0)
A	1.10.246.80	Mainly Alpha	Orthogonal Bundle	Serum Albumin	Chain A, Domain 1	CATH (4.3.0)
B	3.30.460.10	Alpha Beta	2-Layer Sandwich	Beta Polymerase	domain 2	CATH (4.3.0)
B	1.10.110.30	Mainly Alpha	Orthogonal Bundle	Hydrophobic Seed Protein		CATH (4.3.0)
B	1.20.58.560	Mainly Alpha	Up-down Bundle	Methane Monooxygenase Hydroxylase	Chain G, domain 1	CATH (4.3.0)
B	1.10.246.80	Mainly Alpha	Orthogonal Bundle	Serum Albumin	Chain A, Domain 1	CATH (4.3.0)

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	tRNA CCA-adding enzyme	cation binding magnesium ion binding ion binding binding metal ion binding small molecule binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding purine nucleotide binding anion binding adenyl nucleotide binding cation binding magnesium ion binding ion binding binding metal ion binding small molecule binding adenyl ribonucleotide binding nucleotide binding purine ribonucleotide binding purine ribonucleoside triphosphate binding nucleoside phosphate binding purine nucleotide binding anion binding adenyl nucleotide binding ATP binding heterocyclic compound binding carbohydrate derivative binding ribonucleotide binding cytidylyltransferase activity catalytic activity, acting on RNA catalytic activity, acting on a nucleic acid catalytic activity, acting on a tRNA transferase activity nucleotidyltransferase activity catalytic activity CCA tRNA nucleotidyltransferase activity transferase activity, transferring phosphorus-containing groups CCACCA tRNA nucleotidyltransferase activity RNA binding tRNA binding nucleic acid binding	nucleobase-containing compound metabolic process RNA metabolic process metabolic process cellular process primary metabolic process macromolecule metabolic process RNA repair nucleic acid metabolic process tRNA 3'-terminal CCA addition gene expression nucleobase-containing compound biosynthetic process macromolecule biosynthetic process tRNA metabolic process RNA biosynthetic process nucleobase-containing compound metabolic process RNA metabolic process metabolic process cellular process primary metabolic process macromolecule metabolic process RNA repair nucleic acid metabolic process tRNA 3'-terminal CCA addition gene expression nucleobase-containing compound biosynthetic process macromolecule biosynthetic process tRNA metabolic process RNA biosynthetic process nucleic acid biosynthetic process biosynthetic process tRNA processing RNA 3'-end processing RNA processing tRNA 3'-end processing	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR023068	CCA-adding enzyme, firmicutes	Family
A, B	IPR050264	Bacterial CCA-adding enzyme type 3 subfamily	Family
A, B	IPR032810	CCA-adding enzyme, C-terminal	Domain
A, B	IPR043519	Nucleotidyltransferase superfamily	Homologous Superfamily
A, B	IPR002646	Poly A polymerase, head domain	Domain
A, B	IPR032828	tRNA nucleotidyltransferase/poly(A) polymerase, RNA and SrmB- binding domain	Domain

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.