1ML1

PROTEIN ENGINEERING WITH MONOMERIC TRIOSEPHOSPHATE ISOMERASE: THE MODELLING AND STRUCTURE VERIFICATION OF A SEVEN RESIDUE LOOP

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d1ml1a_	Alpha and beta proteins (a/b)	TIM beta/alpha-barrel	Triosephosphate isomerase (TIM)	Triosephosphate isomerase (TIM)	Triosephosphate isomerase	(Trypanosoma brucei brucei ) [TaxId: 5702 ],	SCOPe (2.08)
B [auth C]	d1ml1c_	Alpha and beta proteins (a/b)	TIM beta/alpha-barrel	Triosephosphate isomerase (TIM)	Triosephosphate isomerase (TIM)	Triosephosphate isomerase	(Trypanosoma brucei brucei ) [TaxId: 5702 ],	SCOPe (2.08)
D [auth G]	d1ml1g_	Alpha and beta proteins (a/b)	TIM beta/alpha-barrel	Triosephosphate isomerase (TIM)	Triosephosphate isomerase (TIM)	Triosephosphate isomerase	(Trypanosoma brucei brucei ) [TaxId: 5702 ],	SCOPe (2.08)
E [auth I]	d1ml1i_	Alpha and beta proteins (a/b)	TIM beta/alpha-barrel	Triosephosphate isomerase (TIM)	Triosephosphate isomerase (TIM)	Triosephosphate isomerase	(Trypanosoma brucei brucei ) [TaxId: 5702 ],	SCOPe (2.08)
C [auth E]	d1ml1e_	Alpha and beta proteins (a/b)	TIM beta/alpha-barrel	Triosephosphate isomerase (TIM)	Triosephosphate isomerase (TIM)	Triosephosphate isomerase	(Trypanosoma brucei brucei ) [TaxId: 5702 ],	SCOPe (2.08)
F [auth K]	d1ml1k_	Alpha and beta proteins (a/b)	TIM beta/alpha-barrel	Triosephosphate isomerase (TIM)	Triosephosphate isomerase (TIM)	Triosephosphate isomerase	(Trypanosoma brucei brucei ) [TaxId: 5702 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Triosephosphate isomerase (TIM)	8040644	3000190	SCOP2B (2022-06-29)
B [auth C]	SCOP2B Superfamily	Triosephosphate isomerase (TIM)	8040644	3000190	SCOP2B (2022-06-29)
D [auth G]	SCOP2B Superfamily	Triosephosphate isomerase (TIM)	8040644	3000190	SCOP2B (2022-06-29)
E [auth I]	SCOP2B Superfamily	Triosephosphate isomerase (TIM)	8040644	3000190	SCOP2B (2022-06-29)
C [auth E]	SCOP2B Superfamily	Triosephosphate isomerase (TIM)	8040644	3000190	SCOP2B (2022-06-29)
F [auth K]	SCOP2B Superfamily	Triosephosphate isomerase (TIM)	8040644	3000190	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	TIM	e1ml1A1	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: TIM	ECOD (1.6)
B [auth C]	TIM	e1ml1C1	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: TIM	ECOD (1.6)
D [auth G]	TIM	e1ml1G1	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: TIM	ECOD (1.6)
E [auth I]	TIM	e1ml1I1	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: TIM	ECOD (1.6)
C [auth E]	TIM	e1ml1E1	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: TIM	ECOD (1.6)
F [auth K]	TIM	e1ml1K1	A: a/b barrels	X: TIM beta/alpha-barrel	H: TIM barrels (From Topology)	T: TIM barrels	F: TIM	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	3.20.20.70	Alpha Beta	Alpha-Beta Barrel	TIM Barrel	Aldolase class I	CATH (4.3.0)
B [auth C]	3.20.20.70	Alpha Beta	Alpha-Beta Barrel	TIM Barrel	Aldolase class I	CATH (4.3.0)
D [auth G]	3.20.20.70	Alpha Beta	Alpha-Beta Barrel	TIM Barrel	Aldolase class I	CATH (4.3.0)
E [auth I]	3.20.20.70	Alpha Beta	Alpha-Beta Barrel	TIM Barrel	Aldolase class I	CATH (4.3.0)
C [auth E]	3.20.20.70	Alpha Beta	Alpha-Beta Barrel	TIM Barrel	Aldolase class I	CATH (4.3.0)
F [auth K]	3.20.20.70	Alpha Beta	Alpha-Beta Barrel	TIM Barrel	Aldolase class I	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B [auth C], C [auth E], D [auth G], E [auth I], A, B [auth C], C [auth E], D [auth G], E [auth I], F [auth K]	PF00121	Triosephosphate isomerase (TIM)	Triosephosphate isomerase	Triosephosphate isomerase (EC:5.3.1.1) (TIM) [1] is the glycolytic enzyme that catalyses the reversible interconversion of glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. TIM plays an important role in several metabolic pathways and is ess ...	Triosephosphate isomerase (EC:5.3.1.1) (TIM) [1] is the glycolytic enzyme that catalyses the reversible interconversion of glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. TIM plays an important role in several metabolic pathways and is essential for efficient energy production, present in eukaryotes and prokaryotes. TIM is a dimer of identical subunits, each of which is made up of about 250 amino-acid residues. A glutamic acid residue is involved in the catalytic mechanism [2,3]. The tertiary structure of TIM has eight beta/alpha motifs folded into a barrel structure [4]. The sequence around the active site residue is perfectly conserved in all known TIM's. Deficiencies in TIM are associated with haemolytic anaemia coupled with a progressive, severe neurological disorder [5].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B [auth C], C [auth E], D [auth G], E [auth I], A, B [auth C], C [auth E], D [auth G], E [auth I], F [auth K]	TRIOSEPHOSPHATE ISOMERASE	isomerase activity intramolecular oxidoreductase activity, interconverting aldoses and ketoses intramolecular oxidoreductase activity triose-phosphate isomerase activity catalytic activity	ribonucleotide catabolic process pyridine nucleotide catabolic process oxoacid metabolic process carbohydrate catabolic process carbohydrate derivative metabolic process organophosphate catabolic process nucleoside phosphate catabolic process organic acid metabolic process nucleobase-containing compound metabolic process glycolytic process phosphate-containing compound metabolic process pyridine-containing compound metabolic process small molecule metabolic process purine ribonucleotide catabolic process ribonucleotide catabolic process pyridine nucleotide catabolic process oxoacid metabolic process carbohydrate catabolic process carbohydrate derivative metabolic process organophosphate catabolic process nucleoside phosphate catabolic process organic acid metabolic process nucleobase-containing compound metabolic process glycolytic process phosphate-containing compound metabolic process pyridine-containing compound metabolic process small molecule metabolic process purine ribonucleotide catabolic process phosphorus metabolic process pyridine nucleotide metabolic process nucleoside phosphate metabolic process nicotinamide nucleotide metabolic process pyruvate metabolic process purine nucleoside diphosphate metabolic process primary metabolic process purine-containing compound catabolic process nucleotide metabolic process nucleobase-containing compound catabolic process nucleoside diphosphate metabolic process purine nucleoside diphosphate catabolic process nucleoside diphosphate catabolic process metabolic process nucleoside triphosphate metabolic process monocarboxylic acid metabolic process purine ribonucleotide metabolic process pyridine-containing compound catabolic process ribose phosphate metabolic process catabolic process ATP metabolic process purine nucleotide metabolic process ribonucleotide metabolic process nucleobase-containing small molecule metabolic process purine ribonucleoside triphosphate metabolic process ribonucleoside diphosphate metabolic process ribonucleoside diphosphate catabolic process nucleotide catabolic process ribonucleoside triphosphate metabolic process purine nucleoside triphosphate metabolic process purine nucleotide catabolic process carbohydrate metabolic process carbohydrate derivative catabolic process ADP catabolic process carboxylic acid metabolic process generation of precursor metabolites and energy purine-containing compound metabolic process ADP metabolic process organophosphate metabolic process purine ribonucleoside diphosphate metabolic process cellular process purine ribonucleoside diphosphate catabolic process monosaccharide metabolic process small molecule biosynthetic process carbohydrate biosynthetic process hexose metabolic process monosaccharide biosynthetic process gluconeogenesis hexose biosynthetic process biosynthetic process glucose metabolic process polyol metabolic process alcohol catabolic process organic hydroxy compound metabolic process polyol catabolic process alcohol metabolic process alditol catabolic process glycerol metabolic process alditol metabolic process organic hydroxy compound catabolic process glycerol catabolic process small molecule catabolic process carbohydrate derivative biosynthetic process glyceraldehyde-3-phosphate metabolic process aldehyde biosynthetic process aldehyde metabolic process organophosphate biosynthetic process glyceraldehyde-3-phosphate biosynthetic process	cellular anatomical structure intracellular anatomical structure cytoplasm cytosol membrane-bounded organelle intracellular organelle organelle peroxisome microbody glycosome intracellular membrane-bounded organelle

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B [auth C], C [auth E], D [auth G], E [auth I], A, B [auth C], C [auth E], D [auth G], E [auth I], F [auth K]	IPR035990	Triosephosphate isomerase superfamily	Homologous Superfamily
A, B [auth C], C [auth E], D [auth G], E [auth I], A, B [auth C], C [auth E], D [auth G], E [auth I], F [auth K]	IPR000652	Triosephosphate isomerase	Family
A, B [auth C], C [auth E], D [auth G], E [auth I], A, B [auth C], C [auth E], D [auth G], E [auth I], F [auth K]	IPR020861	Triosephosphate isomerase, active site	Active Site
A, B [auth C], C [auth E], D [auth G], E [auth I], A, B [auth C], C [auth E], D [auth G], E [auth I], F [auth K]	IPR013785	Aldolase-type TIM barrel	Homologous Superfamily
A, B [auth C], C [auth E], D [auth G], E [auth I], A, B [auth C], C [auth E], D [auth G], E [auth I], F [auth K]	IPR022896	Triosephosphate isomerase, bacterial/eukaryotic	Family

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.