2NR4 | pdb_00002nr4

Crystal structure of FMN-bound protein MM1853 from Methanosarcina mazei, Pfam DUF447

External Resource: Annotation

Domain Annotation: SCOP/SCOPe Classification
Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP/SCOPe Classification SCOP-e Database Homepage

Chains	Domain Info	Class	Fold	Superfamily	Family	Domain	Species	Provenance Source (Version)
A	d2nr4a1	All beta proteins	Split barrel-like	FMN-binding split barrel	MTH863-like	Hypothetical protein MM1853	(Methanosarcina mazei ) [TaxId: 2209 ],	SCOPe (2.08)
B	d2nr4b_	All beta proteins	Split barrel-like	FMN-binding split barrel	MTH863-like	Hypothetical protein MM1853	(Methanosarcina mazei ) [TaxId: 2209 ],	SCOPe (2.08)

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2 Family	MTH863-like	8022452	4001792	SCOP2 (2022-06-29)
A	SCOP2 Superfamily	FMN-binding split barrel	8034832	3000856	SCOP2 (2022-06-29)
B	SCOP2B Superfamily	FMN-binding split barrel	8034832	3000856	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	DUF447_N	e2nr4A1	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: FMN-binding split barrel	F: DUF447_N	ECOD (1.6)
A	DUF447_C	e2nr4A2	A: alpha bundles	X: Spectrin repeat-like	H: MTH_863 C-terminal domain-like (From Topology)	T: MTH_863 C-terminal domain-like	F: DUF447_C	ECOD (1.6)
B	DUF447_N	e2nr4B1	A: beta barrels	X: cradle loop barrel	H: RIFT-related	T: FMN-binding split barrel	F: DUF447_N	ECOD (1.6)
B	DUF447_C	e2nr4B2	A: alpha bundles	X: Spectrin repeat-like	H: MTH_863 C-terminal domain-like (From Topology)	T: MTH_863 C-terminal domain-like	F: DUF447_C	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	2.30.110.10	Mainly Beta	Roll	Pnp Oxidase	Chain A	CATH (4.3.0)
A	1.20.58.290	Mainly Alpha	Up-down Bundle	Methane Monooxygenase Hydroxylase	Chain G, domain 1	CATH (4.3.0)
B	2.30.110.10	Mainly Beta	Roll	Pnp Oxidase	Chain A	CATH (4.3.0)
B	1.20.58.290	Mainly Alpha	Up-down Bundle	Methane Monooxygenase Hydroxylase	Chain G, domain 1	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF04289	Protein of unknown function (DUF447) N-terminal domain (DUF447_N)	Protein of unknown function (DUF447) N-terminal domain	Archaeal protein of unknown function. A fungal member UniProtKB:M2LN89 is clearly a Flavine-reductase enzyme by homology, and UniProtKB:O28442 has been shown to bind riboflavin 5'-phosphate (unpublished structural Xray analysis).	Domain
A, B	PF20766	Protein of unknown function (DUF447) (DUF447_C)	Protein of unknown function (DUF447)	This entry represents a spectrin-like domain found in the DUF447 family of proteins.	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	Conserved hypothetical protein	binding nucleoside phosphate binding nucleotide binding heterocyclic compound binding small molecule binding	-	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR012349	FMN-binding split barrel	Homologous Superfamily
A, B	IPR007386	DUF447, N-terminal domain	Domain
A, B	IPR049288	DUF447, C-terminal domain	Domain

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.