4BET

Crystal structure of the Legionella pneumophila FIC domain-containing effector AnkX protein (inactive H229A mutant) in complex with cytidine-diphosphate-choline

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Ankyrin repeat	8089795	3000154	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	Fic-like	8089796	3001922	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Fic-like	8089796	3001922	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Ankyrin repeat	8089795	3000154	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Fic_1	e4betA3	A: alpha bundles	X: Fic-like (From Topology)	H: Fic-like (From Topology)	T: Fic-like	F: Fic_1	ECOD (1.6)
A	Ank_2	e4betA2	A: alpha superhelices	X: Repetitive alpha hairpins	H: Ankyrin repeat (From Topology)	T: Ankyrin repeat	F: Ank_2	ECOD (1.6)
A	EUF08480	e4betA1	A: a+b two layers	X: Insertion domain in phosphocholine transferase AnkX (From Topology)	H: Insertion domain in phosphocholine transferase AnkX (From Topology)	T: Insertion domain in phosphocholine transferase AnkX	F: EUF08480	ECOD (1.6)
B	Fic_1	e4betB1	A: alpha bundles	X: Fic-like (From Topology)	H: Fic-like (From Topology)	T: Fic-like	F: Fic_1	ECOD (1.6)
B	Ank_2	e4betB3	A: alpha superhelices	X: Repetitive alpha hairpins	H: Ankyrin repeat (From Topology)	T: Ankyrin repeat	F: Ank_2	ECOD (1.6)
B	EUF08480	e4betB2	A: a+b two layers	X: Insertion domain in phosphocholine transferase AnkX (From Topology)	H: Insertion domain in phosphocholine transferase AnkX (From Topology)	T: Insertion domain in phosphocholine transferase AnkX	F: EUF08480	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	1.25.40.20	Mainly Alpha	Alpha Horseshoe	Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat	Ankyrin repeat-containing domain	CATH (4.3.0)
B	1.25.40.20	Mainly Alpha	Alpha Horseshoe	Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat	Ankyrin repeat-containing domain	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B	PF12796	Ankyrin repeats (3 copies) (Ank_2)	Ankyrin repeats (3 copies)	-	Repeat
A, B	PF22170	Phosphocholine transferase AnkX insertion domain (AnkX_ins)	Phosphocholine transferase AnkX insertion domain	This entry represents the insertion domain of AnkX, a Legionella pneumophila PC transferase that mediates the recruitment of Rab1 to the cytosolic face of the Legionella-containing vacuole (LCVs). It is a virulence factor that modifies host RAB1 (RAB ...	This entry represents the insertion domain of AnkX, a Legionella pneumophila PC transferase that mediates the recruitment of Rab1 to the cytosolic face of the Legionella-containing vacuole (LCVs). It is a virulence factor that modifies host RAB1 (RAB1A, RAB1B, or RAB1C) and RAB35 leading to the displacement of GDP dissociation inhibitors (GDI) and impairs accessibility to the GTPase effector LepB. It consists of two distinct structural units. The N-terminal contains a FIC domain, which encompasses the CDP-choline binding pocket and a C-terminal ankyrin repeats-containing domain [1,2]. Unlike other FIC enzymes, AnkX comprises an additional insert domain located in the conserved beta-hairpin which consists of alpha-helices and beta-sheets that protrudes from the enzyme and adapts a rigid, thorn-like structure [2].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B	PHOSPHOCHOLINE TRANSFERASE ANKX	phosphocholine transferase activity transferase activity catalytic activity phosphotransferase activity, alcohol group as acceptor transferase activity, transferring phosphorus-containing groups	regulation of molecular function regulation of catalytic activity regulation of hydrolase activity biological regulation regulation of GTPase activity	cellular anatomical structure extracellular region host cellular component host intracellular part host cell cytoplasm host cell part host intracellular region

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B	IPR054037	Phosphocholine transferase AnkX, insertion domain	Domain
A, B	IPR002110	Ankyrin repeat	Repeat
A, B	IPR036770	Ankyrin repeat-containing domain superfamily	Homologous Superfamily
A, B	IPR036597	Fido-like domain superfamily	Homologous Superfamily
A, B	IPR050016	Dot/Icm T4SS effector AnkX/LegA8	Family
A, B	IPR003812	Fido domain	Domain

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.