4D7Q | pdb_00004d7q

Crystal structure of a chimeric protein with the Sec7 domain of Legionella pneumophila RalF and the capping domain of Rickettsia prowazekii RalF

External Resource: Annotation

Domain Annotation: SCOP2 Classification
Domain Annotation: ECOD Classification
Domain Annotation: CATH
Protein Family Annotation
Gene Ontology: Gene Product Annotation
InterPro: Protein Family Classification

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
A	SCOP2B Superfamily	Sec7 domain-like	8044149	3001279	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
A	Sec7	e4d7qA1	A: alpha arrays	X: Sec7 domain (From Topology)	H: Sec7 domain (From Topology)	T: Sec7 domain	F: Sec7	ECOD (1.6)
A	EUF08414	e4d7qA2	A: a+b two layers	X: TBP-like	H: RalF, C-terminal domain (From Topology)	T: RalF, C-terminal domain	F: EUF08414	ECOD (1.6)

Domain Annotation: CATH CATH Database Homepage

Chain	Domain	Class	Architecture	Topology	Homology	Provenance Source (Version)
A	1.10.220.20	Mainly Alpha	Orthogonal Bundle	Annexin V	domain 1	CATH (4.3.0)
A	1.10.1000.11	Mainly Alpha	Orthogonal Bundle	Arf Nucleotide-binding Site Opener	domain 2	CATH (4.3.0)
A	3.30.310.140	Alpha Beta	2-Layer Sandwich	TATA-Binding Protein	sec7 domains	CATH (4.3.0)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A	PF18248	RalF C-terminal Sec-7 capping domain (RalF_SCD)	RalF C-terminal Sec-7 capping domain	This is the C-terminal domain of RalF protein present in Legionella pneumophilia. RalF is secreted into host cytosol via the Dot/Icm type IV transporter where it acts to recruit ADP-ribosylation factor (Arf) to pathogen-containing phagosomes in the e ...	This is the C-terminal domain of RalF protein present in Legionella pneumophilia. RalF is secreted into host cytosol via the Dot/Icm type IV transporter where it acts to recruit ADP-ribosylation factor (Arf) to pathogen-containing phagosomes in the establishment of a replicative organelle. This domain forms a cap over the active site in the Sec7 domain and so is referred to as the Sec7-capping domain [1].	Domain
A	PF01369	Sec7 domain (Sec7)	Sec7 domain	The Sec7 domain is a guanine-nucleotide-exchange-factor (GEF) for the Pfam:PF00025 family [2].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A	RALF, PROLINE/BETAINE TRANSPORTER	enzyme regulator activity guanyl nucleotide binding GTP binding nucleotide binding hydrolase activity guanyl ribonucleotide binding GDP binding purine ribonucleotide binding ion binding ribonucleoside triphosphate phosphatase activity anion binding molecular function regulator activity heterocyclic compound binding carbohydrate derivative binding enzyme regulator activity guanyl nucleotide binding GTP binding nucleotide binding hydrolase activity guanyl ribonucleotide binding GDP binding purine ribonucleotide binding ion binding ribonucleoside triphosphate phosphatase activity anion binding molecular function regulator activity heterocyclic compound binding carbohydrate derivative binding hydrolase activity, acting on acid anhydrides catalytic activity ribonucleotide binding pyrophosphatase activity GTPase activity binding nucleoside-triphosphatase regulator activity GTPase regulator activity purine ribonucleoside triphosphate binding guanyl-nucleotide exchange factor activity nucleoside phosphate binding purine nucleotide binding hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides small molecule binding	cellular response to stimulus regulation of biological process regulation of signaling regulation of cellular process small GTPase-mediated signal transduction response to stimulus signaling regulation of small GTPase mediated signal transduction biological regulation regulation of signal transduction ARF protein signal transduction intracellular signal transduction cell communication regulation of intracellular signal transduction regulation of response to stimulus cellular response to stimulus regulation of biological process regulation of signaling regulation of cellular process small GTPase-mediated signal transduction response to stimulus signaling regulation of small GTPase mediated signal transduction biological regulation regulation of signal transduction ARF protein signal transduction intracellular signal transduction cell communication regulation of intracellular signal transduction regulation of response to stimulus regulation of ARF protein signal transduction intracellular signaling cassette regulation of cell communication cellular process signal transduction	-

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A	IPR023394	Sec7, C-terminal domain superfamily	Homologous Superfamily
A	IPR040917	RalF, C-terminal Sec-7 capping domain	Domain
A	IPR035999	Sec7 domain superfamily	Homologous Superfamily
A	IPR000904	Sec7 domain	Domain
A	IPR038075	RalF, C-terminal domain superfamily	Homologous Superfamily
A	IPR023394	Sec7, C-terminal domain superfamily	Homologous Superfamily
A	IPR040917	RalF, C-terminal Sec-7 capping domain	Domain
A	IPR035999	Sec7 domain superfamily	Homologous Superfamily
A	IPR000904	Sec7 domain	Domain
A	IPR038075	RalF, C-terminal domain superfamily	Homologous Superfamily

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.