Annotations: 6PSN

Domain Annotation: SCOP2 Classification SCOP2 Database Homepage

Chains	Type	Family Name	Domain Identifier	Family Identifier	Provenance Source (Version)
G	SCOP2B Superfamily	Anthrax PA domain 3-like	8054212	3000301	SCOP2B (2022-06-29)
G	SCOP2B Superfamily	GLEYA/PA14-like	8054210	3002282	SCOP2B (2022-06-29)
G	SCOP2B Superfamily	FIP-like	8054214	3002056	SCOP2B (2022-06-29)
F	SCOP2B Superfamily	Anthrax PA domain 3-like	8054212	3000301	SCOP2B (2022-06-29)
F	SCOP2B Superfamily	FIP-like	8054214	3002056	SCOP2B (2022-06-29)
F	SCOP2B Superfamily	GLEYA/PA14-like	8054210	3002282	SCOP2B (2022-06-29)
E	SCOP2B Superfamily	Anthrax PA domain 3-like	8054212	3000301	SCOP2B (2022-06-29)
E	SCOP2B Superfamily	GLEYA/PA14-like	8054210	3002282	SCOP2B (2022-06-29)
E	SCOP2B Superfamily	FIP-like	8054214	3002056	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	FIP-like	8054214	3002056	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	GLEYA/PA14-like	8054210	3002282	SCOP2B (2022-06-29)
D	SCOP2B Superfamily	Anthrax PA domain 3-like	8054212	3000301	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	FIP-like	8054214	3002056	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	Anthrax PA domain 3-like	8054212	3000301	SCOP2B (2022-06-29)
C	SCOP2B Superfamily	GLEYA/PA14-like	8054210	3002282	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	FIP-like	8054214	3002056	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	GLEYA/PA14-like	8054210	3002282	SCOP2B (2022-06-29)
B	SCOP2B Superfamily	Anthrax PA domain 3-like	8054212	3000301	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	Anthrax PA domain 3-like	8054212	3000301	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	FIP-like	8054214	3002056	SCOP2B (2022-06-29)
A	SCOP2B Superfamily	GLEYA/PA14-like	8054210	3002282	SCOP2B (2022-06-29)
H [auth L]	SCOP2B Superfamily	Metalloproteases (zincins), catalytic domain	8036789	3001975	SCOP2B (2022-06-29)

Domain Annotation: ECOD Classification ECOD Database Homepage

Chains	Family Name	Domain Identifier	Architecture	Possible Homology	Homology	Topology	Family	Provenance Source (Version)
G	Binary_toxB_2	e6psnG4	A: beta sandwiches	X: Middle beta-sandwich domain in anthrax protective antigen (From Topology)	H: Middle beta-sandwich domain in anthrax protective antigen (From Topology)	T: Middle beta-sandwich domain in anthrax protective antigen	F: Binary_toxB_2	ECOD (1.6)
G	EUF07840	e6psnG1	A: beta sandwiches	X: Ig-like domain in anthrax protective antigen (From Topology)	H: Ig-like domain in anthrax protective antigen (From Topology)	T: Ig-like domain in anthrax protective antigen	F: EUF07840	ECOD (1.6)
G	Binary_toxB_3	e6psnG3	A: a+b two layers	X: beta-grasp domain in anthrax protective antigen (From Topology)	H: beta-grasp domain in anthrax protective antigen (From Topology)	T: beta-grasp domain in anthrax protective antigen	F: Binary_toxB_3	ECOD (1.6)
G	Binary_toxB	e6psnG2	A: few secondary structure elements	X: Ca-binding domain in anthrax protective antigen (From Topology)	H: Ca-binding domain in anthrax protective antigen (From Topology)	T: Ca-binding domain in anthrax protective antigen	F: Binary_toxB	ECOD (1.6)
F	Binary_toxB_2	e6psnF1	A: beta sandwiches	X: Middle beta-sandwich domain in anthrax protective antigen (From Topology)	H: Middle beta-sandwich domain in anthrax protective antigen (From Topology)	T: Middle beta-sandwich domain in anthrax protective antigen	F: Binary_toxB_2	ECOD (1.6)
F	EUF07840	e6psnF4	A: beta sandwiches	X: Ig-like domain in anthrax protective antigen (From Topology)	H: Ig-like domain in anthrax protective antigen (From Topology)	T: Ig-like domain in anthrax protective antigen	F: EUF07840	ECOD (1.6)
F	Binary_toxB_3	e6psnF3	A: a+b two layers	X: beta-grasp domain in anthrax protective antigen (From Topology)	H: beta-grasp domain in anthrax protective antigen (From Topology)	T: beta-grasp domain in anthrax protective antigen	F: Binary_toxB_3	ECOD (1.6)
F	Binary_toxB	e6psnF2	A: few secondary structure elements	X: Ca-binding domain in anthrax protective antigen (From Topology)	H: Ca-binding domain in anthrax protective antigen (From Topology)	T: Ca-binding domain in anthrax protective antigen	F: Binary_toxB	ECOD (1.6)
E	Binary_toxB_2	e6psnE2	A: beta sandwiches	X: Middle beta-sandwich domain in anthrax protective antigen (From Topology)	H: Middle beta-sandwich domain in anthrax protective antigen (From Topology)	T: Middle beta-sandwich domain in anthrax protective antigen	F: Binary_toxB_2	ECOD (1.6)
E	EUF07840	e6psnE3	A: beta sandwiches	X: Ig-like domain in anthrax protective antigen (From Topology)	H: Ig-like domain in anthrax protective antigen (From Topology)	T: Ig-like domain in anthrax protective antigen	F: EUF07840	ECOD (1.6)
E	Binary_toxB_3	e6psnE4	A: a+b two layers	X: beta-grasp domain in anthrax protective antigen (From Topology)	H: beta-grasp domain in anthrax protective antigen (From Topology)	T: beta-grasp domain in anthrax protective antigen	F: Binary_toxB_3	ECOD (1.6)
E	Binary_toxB	e6psnE1	A: few secondary structure elements	X: Ca-binding domain in anthrax protective antigen (From Topology)	H: Ca-binding domain in anthrax protective antigen (From Topology)	T: Ca-binding domain in anthrax protective antigen	F: Binary_toxB	ECOD (1.6)
D	Binary_toxB_2	e6psnD4	A: beta sandwiches	X: Middle beta-sandwich domain in anthrax protective antigen (From Topology)	H: Middle beta-sandwich domain in anthrax protective antigen (From Topology)	T: Middle beta-sandwich domain in anthrax protective antigen	F: Binary_toxB_2	ECOD (1.6)
D	EUF07840	e6psnD3	A: beta sandwiches	X: Ig-like domain in anthrax protective antigen (From Topology)	H: Ig-like domain in anthrax protective antigen (From Topology)	T: Ig-like domain in anthrax protective antigen	F: EUF07840	ECOD (1.6)
D	Binary_toxB_3	e6psnD2	A: a+b two layers	X: beta-grasp domain in anthrax protective antigen (From Topology)	H: beta-grasp domain in anthrax protective antigen (From Topology)	T: beta-grasp domain in anthrax protective antigen	F: Binary_toxB_3	ECOD (1.6)
D	Binary_toxB	e6psnD1	A: few secondary structure elements	X: Ca-binding domain in anthrax protective antigen (From Topology)	H: Ca-binding domain in anthrax protective antigen (From Topology)	T: Ca-binding domain in anthrax protective antigen	F: Binary_toxB	ECOD (1.6)
C	Binary_toxB_2	e6psnC2	A: beta sandwiches	X: Middle beta-sandwich domain in anthrax protective antigen (From Topology)	H: Middle beta-sandwich domain in anthrax protective antigen (From Topology)	T: Middle beta-sandwich domain in anthrax protective antigen	F: Binary_toxB_2	ECOD (1.6)
C	EUF07840	e6psnC1	A: beta sandwiches	X: Ig-like domain in anthrax protective antigen (From Topology)	H: Ig-like domain in anthrax protective antigen (From Topology)	T: Ig-like domain in anthrax protective antigen	F: EUF07840	ECOD (1.6)
C	Binary_toxB_3	e6psnC3	A: a+b two layers	X: beta-grasp domain in anthrax protective antigen (From Topology)	H: beta-grasp domain in anthrax protective antigen (From Topology)	T: beta-grasp domain in anthrax protective antigen	F: Binary_toxB_3	ECOD (1.6)
C	Binary_toxB	e6psnC4	A: few secondary structure elements	X: Ca-binding domain in anthrax protective antigen (From Topology)	H: Ca-binding domain in anthrax protective antigen (From Topology)	T: Ca-binding domain in anthrax protective antigen	F: Binary_toxB	ECOD (1.6)
B	Binary_toxB_2	e6psnB1	A: beta sandwiches	X: Middle beta-sandwich domain in anthrax protective antigen (From Topology)	H: Middle beta-sandwich domain in anthrax protective antigen (From Topology)	T: Middle beta-sandwich domain in anthrax protective antigen	F: Binary_toxB_2	ECOD (1.6)
B	EUF07840	e6psnB3	A: beta sandwiches	X: Ig-like domain in anthrax protective antigen (From Topology)	H: Ig-like domain in anthrax protective antigen (From Topology)	T: Ig-like domain in anthrax protective antigen	F: EUF07840	ECOD (1.6)
B	Binary_toxB_3	e6psnB4	A: a+b two layers	X: beta-grasp domain in anthrax protective antigen (From Topology)	H: beta-grasp domain in anthrax protective antigen (From Topology)	T: beta-grasp domain in anthrax protective antigen	F: Binary_toxB_3	ECOD (1.6)
B	Binary_toxB	e6psnB2	A: few secondary structure elements	X: Ca-binding domain in anthrax protective antigen (From Topology)	H: Ca-binding domain in anthrax protective antigen (From Topology)	T: Ca-binding domain in anthrax protective antigen	F: Binary_toxB	ECOD (1.6)
A	Binary_toxB_2	e6psnA4	A: beta sandwiches	X: Middle beta-sandwich domain in anthrax protective antigen (From Topology)	H: Middle beta-sandwich domain in anthrax protective antigen (From Topology)	T: Middle beta-sandwich domain in anthrax protective antigen	F: Binary_toxB_2	ECOD (1.6)
A	EUF07840	e6psnA2	A: beta sandwiches	X: Ig-like domain in anthrax protective antigen (From Topology)	H: Ig-like domain in anthrax protective antigen (From Topology)	T: Ig-like domain in anthrax protective antigen	F: EUF07840	ECOD (1.6)
A	Binary_toxB_3	e6psnA1	A: a+b two layers	X: beta-grasp domain in anthrax protective antigen (From Topology)	H: beta-grasp domain in anthrax protective antigen (From Topology)	T: beta-grasp domain in anthrax protective antigen	F: Binary_toxB_3	ECOD (1.6)
A	Binary_toxB	e6psnA3	A: few secondary structure elements	X: Ca-binding domain in anthrax protective antigen (From Topology)	H: Ca-binding domain in anthrax protective antigen (From Topology)	T: Ca-binding domain in anthrax protective antigen	F: Binary_toxB	ECOD (1.6)
H [auth L]	ATLF	e6psnL1	A: mixed a+b and a/b	X: Zincin-like	H: Metalloproteases (zincins) catalytic domain (From Topology)	T: Metalloproteases (zincins) catalytic domain	F: ATLF	ECOD (1.6)

Protein Family Annotation Pfam Database Homepage

Chains	Accession	Name	Description	Comments	Source
A, B, C, D, E A, B, C, D, E, F, G	PF17475	Clostridial binary toxin B/anthrax toxin PA domain 2 (Binary_toxB_2)	Clostridial binary toxin B/anthrax toxin PA domain 2	This domain forms the middle beta sandwish domain in anthrax toxin.	Domain
A, B, C, D, E A, B, C, D, E, F, G	PF03495	Clostridial binary toxin B/anthrax toxin PA Ca-binding domain (Binary_toxB)	Clostridial binary toxin B/anthrax toxin PA Ca-binding domain	This domain is a calcium binding domain in the anthrax toxin protective antigen.	Domain
A, B, C, D, E A, B, C, D, E, F, G	PF17476	Clostridial binary toxin B/anthrax toxin PA domain 3 (Binary_toxB_3)	Clostridial binary toxin B/anthrax toxin PA domain 3	This entry represents the beta-grasp domain in anthrax protective antigen.	Domain
A, B, C, D, E A, B, C, D, E, F, G	PF20835	Anthrax protective antigen, immunoglobulin-like domain (PA_Ig-like)	Anthrax protective antigen, immunoglobulin-like domain	Anthrax protective antigen (PA) is one of the three proteins forming the anthrax toxin. It is involved in the first step of toxin entry into host cells, enabling lethal factor (LF) and oedema factor (EF) to cross to the cytosol. PA undergoes cleavage ...	Anthrax protective antigen (PA) is one of the three proteins forming the anthrax toxin. It is involved in the first step of toxin entry into host cells, enabling lethal factor (LF) and oedema factor (EF) to cross to the cytosol. PA undergoes cleavage by Furin to generate PA20 and PA63. The latter, which adopts a ring-shaped heptameric assembly, shows four distinct domains. The fourth domain, a carboxy- terminal receptor-binding domain, shows an initial hairpin and helix, followed by a beta-sandwich with an immunoglobulin-like fold (this entry) [1-5].	Domain

Gene Ontology: Gene Product Annotation Gene Ontology Database Homepage

Chains	Polymer	Molecular Function	Biological Process	Cellular Component
A, B, C, D, E A, B, C, D, E, F, G	Protective antigen	toxin activity cation binding ion binding binding metal ion binding small molecule binding identical protein binding protein binding	cellular component organization cellular component biogenesis cellular component assembly protein-containing complex organization protein complex oligomerization protein-containing complex assembly protein homooligomerization cellular process cellular component organization or biogenesis symbiont-mediated suppression of host signal transduction pathway symbiont-mediated perturbation of host MAPK cascade symbiont-mediated perturbation of host cellular process biological process involved in interspecies interaction between organisms symbiont-mediated perturbation of host process cellular component organization cellular component biogenesis cellular component assembly protein-containing complex organization protein complex oligomerization protein-containing complex assembly protein homooligomerization cellular process cellular component organization or biogenesis symbiont-mediated suppression of host signal transduction pathway symbiont-mediated perturbation of host MAPK cascade symbiont-mediated perturbation of host cellular process biological process involved in interspecies interaction between organisms symbiont-mediated perturbation of host process symbiont-mediated perturbation of host signal transduction pathway modulation of process of another organism symbiont-mediated suppression of host MAPK cascade biological process involved in interaction with host biological process involved in symbiotic interaction	cellular anatomical structure membrane extracellular region host intracellular membrane-bounded organelle host cellular component host intracellular part host cell membrane other organism part host cell cytoplasm part host intracellular organelle host cell endosome membrane host cell cytoplasm host cell part host cell endosome cellular anatomical structure membrane extracellular region host intracellular membrane-bounded organelle host cellular component host intracellular part host cell membrane other organism part host cell cytoplasm part host intracellular organelle host cell endosome membrane host cell cytoplasm host cell part host cell endosome host intracellular region host cell plasma membrane host cell cytosol
H [auth L]	Lethal factor	peptidase activity catalytic activity, acting on a protein metallopeptidase activity hydrolase activity catalytic activity cation binding transition metal ion binding zinc ion binding ion binding binding metal ion binding small molecule binding metalloendopeptidase activity endopeptidase activity peptidase activity catalytic activity, acting on a protein metallopeptidase activity hydrolase activity catalytic activity cation binding transition metal ion binding zinc ion binding ion binding binding metal ion binding small molecule binding metalloendopeptidase activity endopeptidase activity toxin activity	proteolysis metabolic process cellular process primary metabolic process protein metabolic process macromolecule metabolic process	cellular anatomical structure extracellular region host cellular component host intracellular part host cell cytoplasm host cell part host intracellular region host cell cytosol other organism part host cell cytoplasm part

InterPro: Protein Family Classification InterPro Database Homepage

Chains	Accession	Name	Type
A, B, C, D, E A, B, C, D, E, F, G	IPR035331	Clostridial binary toxin B/anthrax toxin PA, domain 3	Domain
A, B, C, D, E A, B, C, D, E, F, G	IPR048853	Anthrax protective antigen, immunoglobulin-like domain	Domain
A, B, C, D, E A, B, C, D, E, F, G	IPR011658	PA14 domain	Domain
A, B, C, D, E A, B, C, D, E, F, G	IPR027439	Protective antigen, heptamerisation domain	Domain
A, B, C, D, E A, B, C, D, E, F, G	IPR035088	Protective antigen, Ca-binding domain	Domain
A, B, C, D, E A, B, C, D, E, F, G	IPR037524	PA14/GLEYA domain	Domain
A, B, C, D, E A, B, C, D, E, F, G	IPR037149	Protective antigen, heptamerisation domain superfamily	Homologous Superfamily
A, B, C, D, E A, B, C, D, E, F, G	IPR003896	Bacterial exotoxin B	Family
A, B, C, D, E A, B, C, D, E, F, G	IPR027441	Protective antigen, domain 4	Homologous Superfamily
H [auth L]	IPR024079	Metallopeptidase, catalytic domain superfamily	Homologous Superfamily
H [auth L]	IPR047568	Anthrax toxin lethal factor-like domain	Domain
H [auth L]	IPR003541	Anthrax toxin, lethal/endema factor	Family
H [auth L]	IPR014781	Anthrax toxin, lethal/endema factor, N-/C-terminal	Domain
H [auth L]	IPR015239	Anthrax toxin lethal factor, central domain	Domain

Membrane Protein Annotation: OPM OPM Database Homepage

Chains	External Link	Type	Class	Superfamily	Family
A, B, C, D, E A, B, C, D, E, F, G, H [auth L]	OPM	Transmembrane	Beta-barrel transmembrane	Pore-forming beta-sheet toxins	Channel-forming Bacillus protective antigen (n=14,S=14)