RETRO-INVERSO ANALOGUE OF THE G-H LOOP OF VP1 IN FOOT-AND-MOUTH-DISEASE (FMD) VIRUS, NMR, 10 STRUCTURES
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | COSY | 90% H2O:10% D2O | 0.1 M PHOSPHATE | 5.7 | 1 atm | 285 | ||
| 2 | TOCSY NOESY | 90% H2O:10% D2O | 0.1 M PHOSPHATE | 5.7 | 1 atm | 285 | ||
| 3 | ROESY | 90% H2O:10% D2O | 0.1 M PHOSPHATE | 5.7 | 1 atm | 285 | ||
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Bruker | DRX400 | 400 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| TORSION ANGLE DYNAMICS, ANNEALING, MINIMIZATION | RESTRAINT ENERGY MINIMIZATION | DYANA |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | LEAST RESTRAINTS VIOLATIONS, TARGET FUNCTION |
| Conformers Calculated Total Number | 50 |
| Conformers Submitted Total Number | 10 |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | refinement | DYANA | 1.4 | GUNTERT,WUTHRICH |
| 2 | structure solution | DYANA | 1.4 | |
