J-DOMAIN (RESIDUES 1-77) OF THE ESCHERICHIA COLI N-TERMINAL FRAGMENT (RESIDUES 1-104) OF THE MOLECULAR CHAPERONE DNAJ, NMR, 20 STRUCTURES
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | SEE PAPER *JRNL* | 2MM, 10% D2O IN H2O | 50 mM PHOSPHATE | 6.0 | 303 | |||
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Varian | UNITY | 600 |
| 2 | GE | OMEGA | 500 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| DG/SIMULATED ANNEALING | REFINEMENT DETAILS ARE IN THE PRIMARY JOURNAL REFERENCE | XPLOR |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | ZERO VIOLATIONS/ENERGY (SEE PAPER *JRNL*) |
| Conformers Calculated Total Number | 50 |
| Conformers Submitted Total Number | 20 |
| Additional NMR Experimental Information | |
|---|---|
| Details | THE STRUCTURE WAS DETERMINED USING TRIPLE-RESONANCE NMR SPECTROSCOPY ON 13C, 15N LABELED PROTEIN |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | refinement | XPLOR | BRUNGER | |
| 2 | structure solution | X-PLOR | ||
