SOLUTION STRUCTURE OF THE [AF]-C8-DG ADDUCT POSITIONED OPPOSITE DA AT A TEMPLATE-PRIMER JUNCTION
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | NOESY | H2O, D2O | 100 mM NACL, 10mM PHOSPHATE | 7.0 | 1 atm | 322 | ||
| 2 | COSY | H2O, D2O | 100 mM NACL, 10mM PHOSPHATE | 7.0 | 1 atm | 322 | ||
| 3 | TOCSY | H2O, D2O | 100 mM NACL, 10mM PHOSPHATE | 7.0 | 1 atm | 322 | ||
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Varian | UNITYPLUS | 500 |
| 2 | Varian | UNITYPLUS | 600 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| RELAXATION MATRIX REFINEMENT | REFINEMENT DETAILS CAN BE FOUND IN THE JRNL CITATION ABOVE | X-PLOR |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | ALL CONFORMERS PRESENTED |
| Conformers Calculated Total Number | 6 |
| Conformers Submitted Total Number | 6 |
| Additional NMR Experimental Information | |
|---|---|
| Details | IONIC STRENGTH: 100MM NACL, 10MM PHOSPHATE PRESSURE: 1 ATM |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | refinement | X-PLOR | 3.1 | BRUNGER |
| 2 | structure solution | VNMR | ||
| 3 | structure solution | DUPLEX | ||
| 4 | structure solution | X-PLOR | ||
