IMMUNOGLOBULIN MOTIF DNA-RECOGNITION AND HETERODIMERIZATION FOR THE PEBP2/CBF RUNT-DOMAIN
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | ALL STANDARD | 96% H2O/4% D2O | 10mM | 6.8 | 1 atm | 310 | ||
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Bruker | DMX500 | 500 |
| 2 | Bruker | DMX600 | 600 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| HYBRID DISTANCE GEOMETRY/SIMULATED ANNEALING | NMRPipe | |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | structures with the least restraint violations |
| Conformers Calculated Total Number | 300 |
| Conformers Submitted Total Number | 43 |
| Additional NMR Experimental Information | |
|---|---|
| Details | ALL STANDARD |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | structure solution | NMRPipe | ||
| 2 | structure solution | PIPP | ||
| 3 | structure solution | STAPP | ||
| 4 | structure solution | X-PLOR | BRUNGER | |
| 5 | refinement | X-PLOR | 3.843 | BRUNGER |
