1EG0 | pdb_00001eg0

FITTING OF COMPONENTS WITH KNOWN STRUCTURE INTO AN 11.5 A CRYO-EM MAP OF THE E.COLI 70S RIBOSOME


ELECTRON MICROSCOPY

Data Collection

Overall
ID #Resolution (High)Resolution (Low)Percent Possible (Observed)Net I Over Average Sigma (I)RedundancyNumber Reflections (All)Number Reflections (Observed)Observed Criterion Sigma (F)Observed Criterion Sigma (I)B (Isotropic) From Wilson Plot
111.5
Sample
E.COLI 70S RIBOSOME
Specimen Preparation
Sample Aggregation StatePARTICLE
Vitrification InstrumentHOMEMADE PLUNGER
Cryogen NameETHANE
Sample Vitrification Details
3D Reconstruction
Reconstruction MethodSINGLE PARTICLE
Number of Particles73523
Reported Resolution (Å)11.5
Resolution Method
Other Details
Refinement Type
Symmetry TypePOINT
Point SymmetryC1
Map-Model Fitting and Refinement
Id1
Refinement SpaceRECIPROCAL
Refinement ProtocolRIGID BODY FIT
Refinement TargetVECTOR R-FACTOR
Overall B Value
Fitting Procedure
DetailsREFINEMENT PROTOCOL--RIGID BODY REFINEMENT DETAILS--ALL COMPONENTS WERE FIT INTO MAP AS RIGID BODIES USING COORDINATES FROM THE REFERRED SOURCES EXCE ...REFINEMENT PROTOCOL--RIGID BODY REFINEMENT DETAILS--ALL COMPONENTS WERE FIT INTO MAP AS RIGID BODIES USING COORDINATES FROM THE REFERRED SOURCES EXCEPT FOR L1, FOR WHICH ABOUT 15-DEGREE ROTATION WAS INTRODUCED BETWEEN THE TWO DOMAINS; FITTING OF PROTEIN S17 IS RELATIVELY UNCERTAIN; CONFORMATIONAL CHANGES OF ANTICODON AND ACCEPTOR REGIONS OF TRNAFMET UPON BINDING TO THE RIBOSOME WERE NOT MODELED.
Data Acquisition
Detector TypeKODAK SO-163 FILM
Electron Dose (electrons/Å**2)10
Imaging Experiment1
Date of Experiment1997-01-01
Temperature (Kelvin)
Microscope ModelFEI/PHILIPS CM200FEG/ST
Minimum Defocus (nm)730
Maximum Defocus (nm)4340
Minimum Tilt Angle (degrees)
Maximum Tilt Angle (degrees)
Nominal CS
Imaging ModeBRIGHT FIELD
Specimen Holder Model
Nominal Magnification50000
Calibrated Magnification
SourceFIELD EMISSION GUN
Acceleration Voltage (kV)200
Imaging Details