GLYTM1BZIP: A CHIMERIC PEPTIDE MODEL OF THE N-TERMINUS OF A RAT SHORT ALPHA TROPOMYOSIN WITH THE N-TERMINUS ENCODED BY EXON 1B
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | TRIPLE RESONANCE | 1-2 mM | 100 mM NaCl, 10 mM phosphate, 10% Deuterium Oxide, pH 6.4 | 0.12 N | 6.4 | atmospheric atm | 281 | |
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Varian | INOVA 500 | 500 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| 1156 CONFORMATIONALLY-RESTRICTING NOE CONSTRAINTS (OUT OF A TOTAL OF 1746 NOE DERIVED DISTANCES) 112 LOOSE DIHEDRAL ANGLE CONSTRAINTS AND FORTY-EIGHT BACKBONE INTRA-HELICAL HYDROGEN BOND CONSTRAINTS WERE UTILILIZED. | REFINEMENT DETAILS CAN BE FOUND IN THE JOURNAL CITATION ABOVE. | DYANA |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | structures with acceptable covalent geometry, structures with the least restraint violations,target function |
| Conformers Calculated Total Number | 200 |
| Conformers Submitted Total Number | 10 |
| Representative Model | 1 (fewest violations) |
| Additional NMR Experimental Information | |
|---|---|
| Details | The structure was determine using triple-resonance NMR spectroscopy. Inter-chain interactions were determined using X-filtered NOESY experiments |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | refinement | DYANA | 1.5 | GUNTERT,WUTHRICH |
| 2 | data analysis | VNMR | 5.3b | VARIAN |
| 3 | structure solution | DYANA | 1.5 | GUNTERT,WUTHRICH |
| 4 | data analysis | AutoAssign | 1.5 | ZIMMERMAN, MONTELIONE |
| 5 | structure solution | AutoStructure | 1.0 | HUANG, MONTELIONE |
| 6 | data analysis | Sparky | 3.74 | GODDARD, KNELLER |
