1IK1 | pdb_00001ik1

Solution Structure of an RNA Hairpin from HRV-14

SOLUTION NMR

NMR Experiment
Experiment	Type	Sample Contents	Solvent	Ionic Strength	pH	Pressure	Temperature (K)	Spectrometer
1	2D NOESY	1.5 mM RNA 14mer; 10 mM NaPi, 90 mM NaCl, 0.1 mM EDTA, pH 7.0	100% D2O	100 mM	7.0	ambient	298
2	DQF-COSY	1.5 mM RNA 14mer; 10 mM NaPi, 90 mM NaCl, 0.1 mM EDTA, pH 7.0	100% D2O	100 mM	7.0	ambient	298
3	TOCSY	1.5 mM RNA 14mer; 10 mM NaPi, 90 mM NaCl, 0.1 mM EDTA, pH 7.0	100% D2O	100 mM	7.0	ambient	298
4	1H,31P-Hetcor	1.5 mM RNA 14mer; 10 mM NaPi, 90 mM NaCl, 0.1 mM EDTA, pH 7.0	100% D2O	100 mM	7.0	ambient	298
5	1H,13C-HMQC	1.5 mM RNA 14mer; 10 mM NaPi, 90 mM NaCl, 0.1 mM EDTA, pH 7.0	100% D2O	100 mM	7.0	ambient	298
6	2D NOESY	0.5 mM RNA 14mer; 10 mM NaPi, 90 mM NaCl, 0.1 mM EDTA, pH 7.0	90% H2O/10% D2O	100 mM	7.0	ambient	273

NMR Spectrometer Information
Spectrometer	Manufacturer	Model	Field Strength
1	Varian	INOVA	600

NMR Refinement
Method	Details	Software
simulated annealing	A total of 172 distance restraints and 40 dihedral angle restraints.	VNMR

NMR Ensemble Information
Conformer Selection Criteria
Conformers Calculated Total Number
Conformers Submitted Total Number	1

Computation: NMR Software
#	Classification	Version	Software Name	Author
1	collection	VNMR
2	processing	NMRPipe
3	data analysis	NMRDraw
4	data analysis	NMRView
5	structure solution	Discover
6	refinement	Discover

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.