1JT8 | pdb_00001jt8

ARCHAEAL INITIATION FACTOR-1A, AIF-1A


SOLUTION NMR
NMR Experiment
ExperimentTypeSample ContentsSolventIonic StrengthpHPressureTemperature (K)Spectrometer
12D_NOESY10 mM sodium phosphate, 300 mM sodium chloride, 5 mM sodium azide, pH 690% H2O/10% D2O300 mM NaCl6.0ambient303
23D_13C-separated_NOESY10 mM sodium phosphate, 300 mM sodium chloride, 5 mM sodium azide, pH 690% H2O/10% D2O300 mM NaCl6.0ambient303
33D_15N-separated_NOESY10 mM sodium phosphate, 300 mM sodium chloride, 5 mM sodium azide, pH 690% H2O/10% D2O300 mM NaCl6.0ambient303
NMR Spectrometer Information
SpectrometerManufacturerModelField Strength
1VarianINOVA500
NMR Refinement
MethodDetailsSoftware
simulated annealing943 NOE-derived distances restraints, 88 dihedral angle restraints, 39 hydrogen bond restraintsX-PLOR
NMR Ensemble Information
Conformer Selection Criteriastructures with acceptable covalent geometry
Conformers Calculated Total Number240
Conformers Submitted Total Number10
Representative Model1 (lowest energy)
Additional NMR Experimental Information
DetailsThis structure was determined using standard NMR techniques.
Computation: NMR Software
#ClassificationVersionSoftware NameAuthor
1refinementX-PLOR3.1Brunger