1K19 | pdb_00001k19

NMR Solution Structure of the Chemosensory Protein CSP2 from Moth Mamestra brassicae


SOLUTION NMR
NMR Experiment
ExperimentTypeSample ContentsSolventIonic StrengthpHPressureTemperature (K)Spectrometer
12D NOESY2 mM of unlabeled protein, in 90% H2O and 10% D2O containing 10mM of sodium phosphate buffer, 25 mM NaCl at pH 6.96.9ambient296
23D_15N-separated_NOESY2 mM of unlabeled protein, in 90% H2O and 10% D2O containing 10mM of sodium phosphate buffer, 25 mM NaCl at pH 6.9
33D_15N-separated_NOESY1 mM of uniformly labeled protein, in 90% H2O and 10% D2O containing 10mM of sodium phosphate buffer, 25 mM NaCl at pH 6.9
4DQF-COSY2 mM of unlabeled protein, in 90% H2O and 10% D2O containing 10mM of sodium phosphate buffer, 25 mM NaCl at pH 6.96.9ambient296
NMR Spectrometer Information
SpectrometerManufacturerModelField Strength
1BrukerDRX500
2VarianINOVA750
NMR Ensemble Information
Conformer Selection Criteriastructures with acceptable covalent geometry, structures with favorable non-bond energy, structures with the least restraint violations, structures with the lowest energy, target function
Conformers Calculated Total Number50
Conformers Submitted Total Number20
Representative Model1 (fewest violations,lowest energy)
Additional NMR Experimental Information
DetailsThis structure was determined using standard 2D homonuclear techniques combined with 3D Heteronuclear techniques.
Computation: NMR Software
#ClassificationVersionSoftware NameAuthor
1collectionXwinNMR2.1
2data analysisXEASY1.3.13
3processingNMRPipe1.8
4structure solutionDIANA2.8
5data analysisDYANA1.5
6processingMOLMOL2.6
7refinementCNS1.0
8data analysisTurbo-FrodoMarch 2001 release a