1MY9 | pdb_00001my9

Solution structure of a K+ cation stabilized dimeric RNA quadruplex containing two G:G(:A):G:G(:A) hexads, G:G:G:G tetrads and UUUU loops


SOLUTION NMR
NMR Experiment
ExperimentTypeSample ContentsSolventIonic StrengthpHPressureTemperature (K)Spectrometer
12D NOESY1.6 mM RNA, 10 mM sodium phosphate buffer (pH 6.5), 16 mM KCl95% H2O/5% D2O16 mM KCl6.5ambient283
22D NOESY1.6 mM RNA, 10 mM sodium phosphate buffer (pH 6.5), 16 mM KCl100% D2O16 mM KCl6.5ambient305.5
32D TOCSY1.6 mM RNA, 10 mM sodium phosphate buffer (pH 6.5), 16 mM KCl100% D2O16 mM KCl6.5ambient305.5
4DQF-COSY1.6 mM RNA, 10 mM sodium phosphate buffer (pH 6.5), 16 mM KCl100% D2O16 mM KCl6.5ambient305.5
NMR Spectrometer Information
SpectrometerManufacturerModelField Strength
1BrukerDRX600
NMR Refinement
MethodDetailsSoftware
simulated annealingX-PLOR
NMR Ensemble Information
Conformer Selection Criteriastructures with the lowest energy
Conformers Calculated Total Number
Conformers Submitted Total Number1
Representative Model1 (lowest energy)
Computation: NMR Software
#ClassificationVersionSoftware NameAuthor
1structure solutionX-PLOR3.851Brunger
2refinementX-PLOR3.851Brunger