1NEE | pdb_00001nee

Structure of archaeal translation factor aIF2beta from Methanobacterium thermoautrophicum


SOLUTION NMR
NMR Experiment
ExperimentTypeSample ContentsSolventIonic StrengthpHPressureTemperature (K)Spectrometer
13D_15N-separated_NOESY~1.0mM aIF2beta, 50mM tris buffer, 300mM NaCl, 50uM ZnCl2, 1mM DTT, pH 6.0, 90% H2O, 10% D2O90% H2O/10% D2O6.01 atm310
2HNHA~1.0mM aIF2beta, 50mM tris buffer, 300mM NaCl, 50uM ZnCl2, 1mM DTT, pH 6.0, 90% H2O, 10% D2O90% H2O/10% D2O6.01 atm310
3HNCACB~1.0mM aIF2beta, 50mM tris buffer, 300mM NaCl, 50uM ZnCl2, 1mM DTT, pH 6.0, 90% H2O, 10% D2O90% H2O/10% D2O6.01 atm310
4CBCA(CO)NH~1.0mM aIF2beta, 50mM tris buffer, 300mM NaCl, 50uM ZnCl2, 1mM DTT, pH 6.0, 90% H2O, 10% D2O90% H2O/10% D2O6.01 atm310
53D_13C-separated_NOESY~1.0mM aIF2beta, 50mM tris buffer, 300mM NaCl, 50uM ZnCl2, 1mM DTT, pH 6.0, 90% H2O, 10% D2O90% H2O/10% D2O
NMR Spectrometer Information
SpectrometerManufacturerModelField Strength
1BrukerDRX500
2VarianUNITYPLUS800
NMR Refinement
MethodDetailsSoftware
The structures are based on 1142 NOE-derived constraints, 118 dihedral angle restraints, 39 hydrogen bonds and 58 NH residual dipolar couplingsXwinNMR
NMR Ensemble Information
Conformer Selection Criteriastructures with acceptable covalent geometry
Conformers Calculated Total Number100
Conformers Submitted Total Number20
Representative Model1 (n/a)
Computation: NMR Software
#ClassificationVersionSoftware NameAuthor
1collectionXwinNMR2.1Bruker
2processingGifa4.31Delsuc
3data analysisXEASY1.3.13Wutrich
4refinementCNS0.9Brunger
5structure solutionARIA1.1Nilges