C-terminal domain of insulin-like growth factor (IGF) binding protein-6: structure and interaction with IGF-II
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | 3D_15N-separated_NOESY | 1 mM protein 10 mM sodium acetate 0.02 % sodium azide | 95% H2O/5% D2O | 10 mM sodium acetate | 4.5 | ambient | 298 | |
| 2 | 3D_13C-separated_NOESY | 1 mM protein 10 mM sodium acetate 0.02 % sodium azide | 95% H2O/5% D2O | 10 mM sodium acetate | 4.5 | ambient | 298 | |
| 3 | HNHA | 1 mM protein 10 mM sodium acetate 0.02 % sodium azide | 95% H2O/5% D2O | 10 mM sodium acetate | 4.5 | ambient | 298 | |
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Bruker | AMX | 500 |
| 2 | Bruker | DRX | 600 |
| 3 | Bruker | 800 | |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| distance geometry simulated annealing | The structures are based on a total of 1067 restraints, 912 are NOE-derived distance constraints, 145 dihedral angle restraints,10 distance restraints from hydrogen bonds. | XwinNMR |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | structures with the lowest energy |
| Conformers Calculated Total Number | 50 |
| Conformers Submitted Total Number | 20 |
| Representative Model | 20 (closest to the average) |
| Additional NMR Experimental Information | |
|---|---|
| Details | This structure was determined using standard 3D heteronuclear techniques. |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | collection | XwinNMR | 3.5 | |
| 2 | data analysis | XEASY | 1.3.13 | |
| 3 | refinement | CYANA | 1.0.3 | |
| 4 | structure solution | X-PLOR | ||
