Features of Reovirus Outer-Capsid Protein mu1 Revealed by Electron and Image Reconstruction of the virion at 7.0-A Resolution
ELECTRON MICROSCOPY
| Sample |
|---|
| HUMAN REOVIRUS VIRIONS (T3D) |
| Specimen Preparation | |
|---|---|
| Sample Aggregation State | PARTICLE |
| Vitrification Instrument | |
| Cryogen Name | ETHANE |
| Sample Vitrification Details | PLUNGED INTO ETHANE |
| 3D Reconstruction | |
|---|---|
| Reconstruction Method | SINGLE PARTICLE |
| Number of Particles | 7939 |
| Reported Resolution (Å) | 7 |
| Resolution Method | |
| Other Details | |
| Refinement Type | |
| Symmetry Type | POINT |
| Point Symmetry | I |
| Map-Model Fitting and Refinement | |||||
|---|---|---|---|---|---|
| Id | 1 (1EJ6, 1JMU, 1N35) | ||||
| Refinement Space | REAL | ||||
| Refinement Protocol | |||||
| Refinement Target | |||||
| Overall B Value | |||||
| Fitting Procedure | |||||
| Details | |||||
| Data Acquisition | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detector Type | KODAK SO-163 FILM | ||||||||
| Electron Dose (electrons/Å**2) | 20 | ||||||||
| Imaging Experiment | 1 | 2 |
|---|---|---|
| Date of Experiment | 1997-12-10 | 2002-04-18 |
| Temperature (Kelvin) | 97 | |
| Microscope Model | FEI/PHILIPS CM200FEG | FEI/PHILIPS CM300FEG/T |
| Minimum Defocus (nm) | 1300 | |
| Maximum Defocus (nm) | 3200 | |
| Minimum Tilt Angle (degrees) | ||
| Maximum Tilt Angle (degrees) | ||
| Nominal CS | 2 | |
| Imaging Mode | BRIGHT FIELD | BRIGHT FIELD |
| Specimen Holder Model | ||
| Nominal Magnification | 38000 | 39200 |
| Calibrated Magnification | 45000 | 47440 |
| Source | FIELD EMISSION GUN | FIELD EMISSION GUN |
| Acceleration Voltage (kV) | 200 | 300 |
| Imaging Details | SAMPLES WERE MAINTAINED AT -176 CELSIUS DEGREE IN THE ELECTRON MICROSCOPES. |
| Image Processing | ||||
|---|---|---|---|---|
| CTF Correction Type | CTF Correction Details | Number of Particles Selected | Particle Selection Details | |
| CTF correction of each particle | ||||
