2FY1 | pdb_00002fy1

A dual mode of RNA recognition by the RBMY protein


SOLUTION NMR
NMR Experiment
ExperimentTypeSample ContentsSolventIonic StrengthpHPressureTemperature (K)Spectrometer
12D NOESY1mM RBMY protein U-15N, 13C; 1mM S1A RNA; 25mM sodium phosphate; 25mM sodium chloride; pH 7; 90% H2O, 10% D2O90% H2O/10% D2O25mM sodium phosphate, 25mM sodium chloride7ambient313
22D TOCSY1mM RBMY protein U-15N; 1mM S1A RNA; 25mM sodium phosphate; 25mM sodium chloride; pH 7; 100% D2O100% D2O25mM sodium phosphate, 25mM sodium chloride7ambient313
33D_13C-separated_NOESY1mM RBMY protein U-15N, 13C; 1mM S1A RNA; 25mM sodium phosphate; 25mM sodium chloride; pH 7; 100% D2O100% D2O25mM sodium phosphate, 25mM sodium chloride7ambient313
43D_15N-separated_NOESY1mM RBMY protein U-15N; 1mM S1A RNA 13C-sugars of the CACAA pentaloop; 25mM sodium phosphate; 25mM sodium chloride; pH 7; 100% D2O100% D2O25mM sodium phosphate, 25mM sodium chloride7ambient313
52D filtered/edited NOESY1mM RBMY protein U-15N; 1mM S1A RNA 15N, 13C-adenine, cytidine; 25mM sodium phosphate; 25mM sodium chloride; pH 7; 100% D2O100% D2O25mM sodium phosphate, 25mM sodium chloride7ambient313
63D filtered 13C-separated NOESY1mM RBMY protein U-15N; 1mM S1A RNA 15N, 13C-guanine, uracil; 25mM sodium phosphate; 25mM sodium chloride; pH 7; 100% D2O100% D2O25mM sodium phosphate, 25mM sodium chloride7ambient313
NMR Spectrometer Information
SpectrometerManufacturerModelField Strength
1BrukerAVANCE900
2BrukerDRX750
3BrukerDRX600
4BrukerDRX500
NMR Ensemble Information
Conformer Selection Criteriastructures with the least restraint violations
Conformers Calculated Total Number30
Conformers Submitted Total Number17
Representative Model1 (fewest violations)
Computation: NMR Software
#ClassificationVersionSoftware NameAuthor
1collectionXwinNMR3.5Bruker
2data analysisSparky3.106Goddard, TD
3refinementAmber7.0