2H7T | pdb_00002h7t

Solution Structure of the C-terminal Domain of Insulin-like Growth Factor Binding Protein 2 (IGFBP-2)

SOLUTION NMR

NMR Experiment
Experiment	Type	Sample Contents	Solvent	Ionic Strength	pH	Pressure	Temperature (K)	Spectrometer
1	3D_15N-separated_NOESY	0.8mM C-BP-2 U-15N; 10mM Na acetate, 95% H2O, 5% D2O	95% H2O/5% D2O	0.01M	6.0	ambient	298
2	HNHA	0.8mM C-BP-2 U-15N; 10mM Na acetate, 95% H2O, 5% D2O	95% H2O/5% D2O	0.01M	6.0	ambient	298
3	3D_13C-separated_NOESY	0.5mM C-BP-2 U-15N, 13C; 10mM Na acetate, 95% H2O, 5% D2O	95% H2O/5% D2O	0.01M	6.0	ambient	298

NMR Spectrometer Information
Spectrometer	Manufacturer	Model	Field Strength
1	Bruker	AVANCE	500
2	Bruker	DRX	600

NMR Refinement
Method	Details	Software
torsion angle dynamics		XwinNMR

NMR Ensemble Information
Conformer Selection Criteria	target function
Conformers Calculated Total Number	200
Conformers Submitted Total Number	20
Representative Model	1 (closest to the average)

Computation: NMR Software
#	Classification	Version	Software Name	Author
1	collection	XwinNMR	3.5	Bruker Biospin
2	data analysis	XEASY	1.3	Bartels
3	structure solution	CYANA	1.0.3	Guentert
4	refinement	OPALp		Koradi

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.