Structure of the N-terminal subdomain of insulin-like growth factor (IGF) binding protein-6 and its interactions with IGFs
SOLUTION NMR
| NMR Experiment | ||||||||
|---|---|---|---|---|---|---|---|---|
| Experiment | Type | Sample Contents | Solvent | Ionic Strength | pH | Pressure | Temperature (K) | Spectrometer |
| 1 | 2D NOESY | 1.1 mM IGFBP-6 | 95% H2O/5% D2O | 10 | 4.3 | ambient | 288 | |
| 2 | 2D TOCSY | 1.1 mM IGFBP-6 | 95% H2O/5% D2O | 10 | 4.3 | ambient | 288 | |
| 3 | 2D DQF-COSY | 1.1 mM IGFBP-6 | 95% H2O/5% D2O | 10 | 4.3 | ambient | 288 | |
| NMR Spectrometer Information | |||
|---|---|---|---|
| Spectrometer | Manufacturer | Model | Field Strength |
| 1 | Bruker | AVANCE | 800 |
| 2 | Bruker | DRX | 600 |
| 3 | Bruker | AVANCE | 500 |
| NMR Refinement | ||
|---|---|---|
| Method | Details | Software |
| simulated annealing | The structures are based on a total of 446 restraints, 428 are noe-derived distance constraints, 18 dihedral angle restraints | X-PLOR NIH |
| NMR Ensemble Information | |
|---|---|
| Conformer Selection Criteria | lowest energy |
| Conformers Calculated Total Number | 50 |
| Conformers Submitted Total Number | 20 |
| Representative Model | 1 (closest to the average) |
| Computation: NMR Software | ||||
|---|---|---|---|---|
| # | Classification | Version | Software Name | Author |
| 1 | structure solution | CYANA | P Guntert, C Mumenthaler and K Wuthrich | |
| 2 | refinement | X-PLOR NIH | CD Schwieters, JJ Kuszewski, N Tjandra and GM Clore | |
