Experiment: 2RQG

SOLUTION NMR

NMR Experiment
Experiment	Type	Sample Contents	Solvent	Ionic Strength	pH	Pressure	Temperature (K)	Spectrometer
1	NOESY	1 mM [U-98% 13C; U-98% 15N] G1 TO S PHASE TRANSITION 1-1, 1 mM [U-98% 13C; U-98% 15N] POLYADENYLATE-BINDING PROTEIN 1-2	93% H2O/7% D2O	0.15M	6.5	AMBIENT	303
2	3D_13C-SEPARATED_ NOESY	1 mM [U-98% 13C; U-98% 15N] G1 TO S PHASE TRANSITION 1-1, 1 mM [U-98% 13C; U-98% 15N] POLYADENYLATE-BINDING PROTEIN 1-2	93% H2O/7% D2O	0.15M	6.5	AMBIENT	303
3	HNHA	1 mM [U-98% 13C; U-98% 15N] G1 TO S PHASE TRANSITION 1-1, 1 mM [U-98% 13C; U-98% 15N] POLYADENYLATE-BINDING PROTEIN 1-2	93% H2O/7% D2O	0.15M	6.5	AMBIENT	303
4	13C-EDITED/13C-FILTERED NOESY	1 mM [U-98% 13C; U-98% 15N] G1 TO S PHASE TRANSITION 1-1, 1 mM [U-98% 13C; U-98% 15N] POLYADENYLATE-BINDING PROTEIN 1-2	93% H2O/7% D2O	0.15M	6.5	AMBIENT	303
5	3D_15N- SEPARATED_NOESY	1 mM [U-98% 13C; U-98% 15N] G1 TO S PHASE TRANSITION 1-1, 1 mM [U-98% 13C; U-98% 15N] POLYADENYLATE-BINDING PROTEIN 1-2	93% H2O/7% D2O	0.15M	6.5	AMBIENT	303

NMR Spectrometer Information
Spectrometer	Manufacturer	Model	Field Strength
1	Bruker	AVANCE	600

NMR Refinement
Method	Details	Software
torsion angle dynamics		Sparky

NMR Ensemble Information
Conformer Selection Criteria	target function
Conformers Calculated Total Number	100
Conformers Submitted Total Number	20
Representative Model	1 (lowest energy)

Additional NMR Experimental Information
Details	THE STRUCTURE WAS DETERMINED USING TRIPLE-RESONANCE NMR SPECTROSCOPY.

Computation: NMR Software
#	Classification	Version	Software Name	Author
1	chemical shift assignment	Sparky	3	Goddard
2	structure solution	CYANA	2.1	Guntert, Mumenthaler and Wuthrich
3	refinement	CYANA	2.1	Guntert, Mumenthaler and Wuthrich

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.