Actin filament model from extended form of acromsomal bundle in the Limulus sperm
ELECTRON CRYSTALLOGRAPHY
Diffraction
| Diffraction Experiment | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ID # | Crystal ID | Scattering Type | Data Collection Temperature | Detector | Detector Type | Details | Collection Date | Monochromator | Protocol | |||||
| 1 | 1 | |||||||||||||
Refinement
| Non-Hydrogen Atoms Used in Refinement | |
|---|---|
| Non-Hydrogen Atoms | Number |
| Protein Atoms | 41062 |
| Nucleic Acid Atoms | |
| Solvent Atoms | |
| Heterogen Atoms | |
| Sample |
|---|
| acrosomal bundle in the Limulus sperm |
| Sample Components |
| Refined (by energy minimization) Actin filament model in the extended form of acromsomal bundle. The starting coordinates of the protomers are the Holmes Actin model from Fiber Diffraction experiment. |
| Specimen Preparation | |
|---|---|
| Sample Aggregation State | 3D ARRAY |
| Vitrification Instrument | HOMEMADE PLUNGER |
| Cryogen Name | ETHANE |
| Sample Vitrification Details | |
| 3D Reconstruction | |
|---|---|
| Reconstruction Method | CRYSTALLOGRAPHY |
| Number of Particles | |
| Reported Resolution (Å) | 9.5 |
| Resolution Method | DIFFRACTION PATTERN/LAYERLINES |
| Other Details | Cross-correlation and merging of crystallographic reflections derived from cryoelectron micrographs of 3D crystalss: application to the Limulus acroso ... |
| Refinement Type | |
| Symmetry Type | 3D CRYSTAL |
| Map-Model Fitting and Refinement | |||||
|---|---|---|---|---|---|
| Id | 1 | ||||
| Refinement Space | REAL | ||||
| Refinement Protocol | AB INITIO MODEL | ||||
| Refinement Target | Best cross-correlation score between model and cryoEM map | ||||
| Overall B Value | |||||
| Fitting Procedure | |||||
| Details | REFINEMENT PROTOCOL--Foldhunter program from EMAN single particle analysis package | ||||
| Data Acquisition | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detector Type | KODAK SO-163 FILM | ||||||||
| Electron Dose (electrons/Å**2) | 15 | ||||||||
| Imaging Experiment | 1 |
|---|---|
| Date of Experiment | |
| Temperature (Kelvin) | 106 |
| Microscope Model | JEOL 4000EX |
| Minimum Defocus (nm) | 800 |
| Maximum Defocus (nm) | 3000 |
| Minimum Tilt Angle (degrees) | |
| Maximum Tilt Angle (degrees) | |
| Nominal CS | |
| Imaging Mode | BRIGHT FIELD |
| Specimen Holder Model | |
| Nominal Magnification | 40000 |
| Calibrated Magnification | |
| Source | LAB6 |
| Acceleration Voltage (kV) | 400 |
| Imaging Details | For more information, please refer to EMDB entry EMD-1088 http://www.ebi.ac.uk/msd-srv/emsearch/atlas/1088_summary.html |
| EM Software | ||
|---|---|---|
| Task | Software Package | Version |
| MODEL FITTING | Foldhunter | |
| RECONSTRUCTION | MRC | |
