Actin filament model in the extended form of acromsomal bundle in the Limulus sperm
ELECTRON MICROSCOPY
Starting Model(s)
| Initial Refinement Model(s) | |||
|---|---|---|---|
| Type | Source | Accession Code | Details |
| experimental model | PDB | 1T44 | |
| experimental model | PDB | 1HLU | |
| experimental model | PDB | 1ATN | |
| experimental model | PDB | 1YAG | |
| experimental model | PDB | 1YVN | |
| experimental model | PDB | 1MDU | |
| experimental model | PDB | 1HIV | |
| experimental model | PDB | 1NWK | |
| experimental model | PDB | 1ESV | |
| Sample |
|---|
| Acrosomal bundle from Limulus sperm |
| Sample Components |
| An actin filament model in the acrosomal bundle refined from multiple actin crystal structures |
| Specimen Preparation | |
|---|---|
| Sample Aggregation State | FILAMENT |
| Vitrification Instrument | HOMEMADE PLUNGER |
| Cryogen Name | ETHANE |
| Sample Vitrification Details | |
| 3D Reconstruction | |
|---|---|
| Reconstruction Method | CRYSTALLOGRAPHY |
| Number of Particles | |
| Reported Resolution (Å) | 9.5 |
| Resolution Method | |
| Other Details | Cross-correlation and merging of crystallographic reflections derived from cryoelectron micrographs of 3D crystalss: application to the Limulus acroso ... |
| Refinement Type | |
| Symmetry Type | 3D CRYSTAL |
| Map-Model Fitting and Refinement | |||||
|---|---|---|---|---|---|
| Id | 1 (1T44, 1HLU, 1ATN, 1YAG, 1YVN, 1MDU, 1HIV, 1NWK, 1ESV) | ||||
| Refinement Space | REAL | ||||
| Refinement Protocol | AB INITIO MODEL | ||||
| Refinement Target | Best cross-correlation score between model and cryoEM map | ||||
| Overall B Value | |||||
| Fitting Procedure | |||||
| Details | REFINEMENT PROTOCOL--Foldhunter program from EMAN single particle analysis package | ||||
| Data Acquisition | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detector Type | KODAK SO-163 FILM | ||||||||
| Electron Dose (electrons/Å**2) | 15 | ||||||||
| Imaging Experiment | 1 |
|---|---|
| Date of Experiment | |
| Temperature (Kelvin) | 106 |
| Microscope Model | JEOL 4000EX |
| Minimum Defocus (nm) | 800 |
| Maximum Defocus (nm) | 3000 |
| Minimum Tilt Angle (degrees) | |
| Maximum Tilt Angle (degrees) | |
| Nominal CS | |
| Imaging Mode | BRIGHT FIELD |
| Specimen Holder Model | |
| Nominal Magnification | 40000 |
| Calibrated Magnification | |
| Source | LAB6 |
| Acceleration Voltage (kV) | 400 |
| Imaging Details | For more information, please refer to EMDB entry EMD-1088 http://www.ebi.ac.uk/msd-srv/emsearch/atlas/1088_summary.html |
| EM Software | ||
|---|---|---|
| Task | Software Package | Version |
| MODEL FITTING | Foldhunter | |
| RECONSTRUCTION | MRC | |
