4ZDT | pdb_00004zdt

Crystal structure of the RING finger domain of Slx1 in complex with the C-terminal domain of Slx4


X-RAY DIFFRACTION

Crystallization

Crystalization Experiments
IDMethodpHTemperatureDetails
1VAPOR DIFFUSION, SITTING DROP6.82911.34M ammonium sulfate, 0.1M Bis-Tris, pH 6.8
Crystal Properties
Matthews coefficientSolvent content
4.0969.97

Crystal Data

Unit Cell
Length ( Å )Angle ( ˚ )
a = 85.233α = 90
b = 85.233β = 90
c = 74.835γ = 90
Symmetry
Space GroupP 41

Diffraction

Diffraction Experiment
ID #Crystal IDScattering TypeData Collection TemperatureDetectorDetector TypeDetailsCollection DateMonochromatorProtocol
11x-ray100CCDADSC QUANTUM 315r2014-06-08MSINGLE WAVELENGTH
Radiation Source
ID #SourceTypeWavelength ListSynchrotron SiteBeamline
1SYNCHROTRONSSRF BEAMLINE BL17U1.28238SSRFBL17U

Data Collection

Overall
ID #Resolution (High)Resolution (Low)Percent Possible (Observed)R Merge I (Observed)R Sym I (Observed)Net I Over Average Sigma (I)RedundancyNumber Reflections (All)Number Reflections (Observed)Observed Criterion Sigma (F)Observed Criterion Sigma (I)B (Isotropic) From Wilson Plot
12501000.1090.10928.914.93612136121-331.9
Highest Resolution Shell
ID #Resolution (High)Resolution (Low)Percent Possible (All)Percent Possible (Observed)R Merge I (Observed)Mean I Over Sigma (Observed)RedundancyNumber Unique Reflections (All)
122.071000.4837.814.7

Refinement

Statistics
Diffraction IDStructure Solution MethodCross Validation methodResolution (High)Resolution (Low)Number Reflections (Observed)Number Reflections (R-Free)Percent Reflections (Observed)R-Factor (Observed)R-Work (Depositor)R-Work (DCC)R-Free (Depositor)R-Free (DCC)R-Free Selection DetailsMean Isotropic B
X-RAY DIFFRACTIONSADTHROUGHOUT238.134383172999.80.216170.215110.210.236310.23RANDOM32.398
Temperature Factor Modeling
Anisotropic B[1][1]Anisotropic B[1][2]Anisotropic B[1][3]Anisotropic B[2][2]Anisotropic B[2][3]Anisotropic B[3][3]
-1.34-1.342.68
RMS Deviations
KeyRefinement Restraint Deviation
r_dihedral_angle_2_deg37.93
r_dihedral_angle_4_deg15.966
r_dihedral_angle_3_deg11.885
r_dihedral_angle_1_deg4.819
r_scangle_it3.462
r_scbond_it2.085
r_mcangle_it1.591
r_angle_refined_deg1.139
r_mcbond_it0.849
r_chiral_restr0.085
RMS Deviations
KeyRefinement Restraint Deviation
r_dihedral_angle_2_deg37.93
r_dihedral_angle_4_deg15.966
r_dihedral_angle_3_deg11.885
r_dihedral_angle_1_deg4.819
r_scangle_it3.462
r_scbond_it2.085
r_mcangle_it1.591
r_angle_refined_deg1.139
r_mcbond_it0.849
r_chiral_restr0.085
r_bond_refined_d0.012
r_gen_planes_refined0.004
r_bond_other_d
r_angle_other_deg
r_gen_planes_other
r_nbd_refined
r_nbd_other
r_nbtor_refined
r_nbtor_other
r_xyhbond_nbd_refined
r_xyhbond_nbd_other
r_metal_ion_refined
r_metal_ion_other
r_symmetry_vdw_refined
r_symmetry_vdw_other
r_symmetry_hbond_refined
r_symmetry_hbond_other
r_symmetry_metal_ion_refined
r_symmetry_metal_ion_other
r_mcbond_other
r_mcangle_other
r_scbond_other
r_scangle_other
r_long_range_B_refined
r_long_range_B_other
r_rigid_bond_restr
r_sphericity_free
r_sphericity_bonded
Non-Hydrogen Atoms Used in Refinement
Non-Hydrogen AtomsNumber
Protein Atoms2217
Nucleic Acid Atoms
Solvent Atoms107
Heterogen Atoms39

Software

Software
Software NamePurpose
REFMACrefinement
SCALEPACKdata reduction
HKL-2000data scaling
PHENIXphasing