Download MendeleyCrystal structures of nitrophorin 4 triple mutant
Issaian, V.A., Weichsel, A., Montfort, W.R.To be published.
4GNW
Crystal structure of nitrophorin 4 triple mutant complex with ammonia
- PDB DOI: https://doi.org/10.2210/pdb4GNW/pdb
- Classification: TRANSPORT PROTEIN
- Organism(s): Rhodnius prolixus
- Expression System: Escherichia coli
- Mutation(s): Yes
- Deposited: 2012-08-17 Released: 2013-09-18
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.15 Å
- R-Value Free: 0.181
- R-Value Work: 0.162
- R-Value Observed: 0.163
Starting Model: experimental
View more details
This is version 1.2 of the entry. See complete history.
Literature
To be published.
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| Nitrophorin-4 | 184 | Rhodnius prolixus | Mutation(s): 3 EC: 1.7.6.1 |  | |
UniProt | |||||
Find proteins for Q94734 (Rhodnius prolixus) Explore Q94734 Go to UniProtKB: Q94734 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | Q94734 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 3 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| HEM Query on HEM | C [auth A], F [auth B] | PROTOPORPHYRIN IX CONTAINING FE C34 H32 Fe N4 O4 KABFMIBPWCXCRK-RGGAHWMASA-L |  | ||
| PO4 Query on PO4 | E [auth A], H [auth B] | PHOSPHATE ION O4 P NBIIXXVUZAFLBC-UHFFFAOYSA-K |  | ||
| NH3 Query on NH3 | D [auth A], G [auth B] | AMMONIA H3 N QGZKDVFQNNGYKY-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.15 Å
- R-Value Free: 0.181
- R-Value Work: 0.162
- R-Value Observed: 0.163
- Space Group: P 31 2 1
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 69.74 | α = 90 |
| b = 69.74 | β = 90 |
| c = 141.676 | γ = 120 |
| Software Name | Purpose |
|---|---|
| Blu-Ice | data collection |
| MOLREP | phasing |
| REFMAC | refinement |
| CrystalClear | data reduction |
| CrystalClear | data scaling |
Entry History
Deposition Data
- Released Date: 2013-09-18 Deposition Author(s): Weichsel, A., Issaian, A.V., Montfort, W.R.
Revision History (Full details and data files)
- Version 1.0: 2013-09-18
Type: Initial release - Version 1.1: 2023-09-13
Changes: Data collection, Database references, Derived calculations, Refinement description - Version 1.2: 2024-11-06
Changes: Structure summary
