Download MendeleyHumanisation of RadA from Pyrococcus furiosus
Fischer, G., Hyvonen, M.To be published.
5JFG
Structure of humanised RadA-mutant humRadA22F in complex with peptide FHTA
- PDB DOI: https://doi.org/10.2210/pdb5JFG/pdb
- Classification: HYDROLASE
- Organism(s): Pyrococcus furiosus, synthetic construct
- Expression System: Escherichia coli BL21(DE3)
- Mutation(s): Yes
- Deposited: 2016-04-19 Released: 2016-10-26
Experimental Data Snapshot
- Method: X-RAY DIFFRACTION
- Resolution: 1.77 Å
- R-Value Free: 0.192
- R-Value Work: 0.174
- R-Value Observed: 0.175
Starting Model: other
View more details
wwPDB Validation 3D Report Full Report
This is version 1.1 of the entry. See complete history.
Literature
Macromolecules
Find similar proteins by:
(by identity cutoff) | 3D Structure
Entity ID: 1 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| DNA repair and recombination protein RadA | 231 | Pyrococcus furiosus | Mutation(s): 11 Gene Names: radA, PF1926 |  | |
UniProt | |||||
Find proteins for O74036 (Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)) Explore O74036 Go to UniProtKB: O74036 | |||||
Entity Groups | |||||
| Sequence Clusters | 30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity | ||||
| UniProt Group | O74036 | ||||
Sequence AnnotationsExpand | |||||
| |||||
Find similar proteins by: Sequence | 3D Structure
Entity ID: 2 | |||||
|---|---|---|---|---|---|
| Molecule | Chains | Sequence Length | Organism | Details | Image |
| PEPTIDE FHTA | 6 | synthetic construct | Mutation(s): 0 |  | |
Sequence AnnotationsExpand | |||||
| |||||
Small Molecules
| Ligands 2 Unique | |||||
|---|---|---|---|---|---|
| ID | Chains | Name / Formula / InChI Key | 2D Diagram | 3D Interactions | |
| DMS Query on DMS | C [auth A], D [auth A], E [auth A] | DIMETHYL SULFOXIDE C2 H6 O S IAZDPXIOMUYVGZ-UHFFFAOYSA-N |  | ||
| CA Query on CA | F [auth A] | CALCIUM ION Ca BHPQYMZQTOCNFJ-UHFFFAOYSA-N |  | ||
Experimental Data & Validation
Experimental Data
- Method: X-RAY DIFFRACTION
- Resolution: 1.77 Å
- R-Value Free: 0.192
- R-Value Work: 0.174
- R-Value Observed: 0.175
- Space Group: P 21 21 21
Unit Cell:
| Length ( Å ) | Angle ( ˚ ) |
|---|---|
| a = 40.515 | α = 90 |
| b = 59.269 | β = 90 |
| c = 88.144 | γ = 90 |
| Software Name | Purpose |
|---|---|
| BUSTER | refinement |
| XDS | data reduction |
| Aimless | data scaling |
| PHASER | phasing |
| Coot | model building |
Entry History
Deposition Data
- Released Date: 2016-10-26 Deposition Author(s): Fischer, G., Marsh, M., Moschetti, T., Sharpe, T., Scott, D., Morgan, M., Ng, H., Skidmore, J., Venkitaraman, A., Abell, C., Blundell, T.L., Hyvonen, M.
Revision History (Full details and data files)
- Version 1.0: 2016-10-26
Type: Initial release - Version 1.1: 2024-05-01
Changes: Data collection, Database references, Refinement description
