5TSY | pdb_00005tsy

Structure of the glycoside hydrolase domain of PelA variant E218A from Pseudomonas aeruginosa

PDB DOI: https://doi.org/10.2210/pdb5TSY/pdb

Classification: HYDROLASE
Organism(s): Pseudomonas aeruginosa PAO1
Expression System: Escherichia coli
Mutation(s): Yes

Deposited: 2016-10-31 Released: 2017-11-08
Deposition Author(s): Baker, P., Pfoh, R., Howell, P.L.
Funding Organization(s): Canadian Institutes of Health Research (CIHR)

Experimental Data Snapshot

Method: X-RAY DIFFRACTION
Resolution: 1.90 Å
R-Value Free:
0.231 (Depositor), 0.230 (DCC)
R-Value Work:
0.184 (Depositor), 0.180 (DCC)
R-Value Observed:
0.186 (Depositor)

Starting Model: experimental
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wwPDB Validation 3D Report Full Report

This is version 1.3 of the entry. See complete history.

Literature

Microbial glycoside hydrolases display cross-kingdom activity against bacterial and fungal biofilms

Snarr, B.D., Baker, P., Bamford, N.C., Sato, Y., Alnabelseya, N., Lui, H., Gravelad, F.N., Ostapska, H., Baistrocchi, S.R., Cerone, R.P., Robinson, H., Filler, S.G., Howell, P.L., Sheppard, D.C.

To be published.

Macromolecule Content

Total Structure Weight: 30.52 kDa
Atom Count: 2,146
Modelled Residue Count: 253
Deposited Residue Count: 278
Unique protein chains: 1

Macromolecules

Find similar proteins by:

(by identity cutoff) | 3D Structure

Entity ID: 1
Molecule	Chains	Sequence Length	Organism	Details	Image
PelA	A	278	Pseudomonas aeruginosa PAO1	Mutation(s): 1 Gene Names: pelA, PA3064
UniProt
Find proteins for Q9HZE4 (Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)) Explore Q9HZE4 Go to UniProtKB: Q9HZE4
Entity Groups
Sequence Clusters	30% Identity50% Identity70% Identity90% Identity95% Identity100% Identity
UniProt Group	Q9HZE4
Sequence Annotations Expand
Reference Sequence LOADING

Experimental Data & Validation

Experimental Data

Method: X-RAY DIFFRACTION
Resolution: 1.90 Å
R-Value Free: 0.231 (Depositor), 0.230 (DCC)
R-Value Work: 0.184 (Depositor), 0.180 (DCC)
R-Value Observed: 0.186 (Depositor)

Space Group: P 2₁ 2₁ 2

Unit Cell:

Length ( Å )	Angle ( ˚ )
a = 65.421	α = 90
b = 84.098	β = 90
c = 47.179	γ = 90

Software Package:

Software Name	Purpose
PHENIX	refinement
SAINT	data reduction
SAINT	data scaling
PHASER	phasing

Structure Validation

View Full Validation Report

Entry History & Funding Information

Deposition Data

Released Date: 2017-11-08

Deposition Author(s):

Funding Organization	Location	Grant Number
Canadian Institutes of Health Research (CIHR)	Canada	43998

Revision History (Full details and data files)

Version 1.0: 2017-11-08
Type: Initial release
Version 1.1: 2018-03-07
Changes: Data collection
Version 1.2: 2020-01-08
Changes: Author supporting evidence
Version 1.3: 2023-10-04
Changes: Data collection, Database references, Refinement description

RCSB PDB Core Operations are funded by the U.S. National Science Foundation (DBI-2321666), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM157729.